SOCS-3基因在Balb/c小鼠脑组织中的表达与脑不对称性

The Heterogeneity of SOCS-3 in The Brain of Balb/c Mice and Brain Lateralization

为探讨细胞因子信号传导抑制因子 3(suppressoofcytokinesignaling 3,SOCS 3)在Balb/c小鼠皮层、海马、下丘脑中的表达及其与脑不对称性的关系 .通过伸爪取食法将Balb/c小鼠分为左利组和右利组 ,取左、右侧皮层 ,左、右侧海马及下丘脑 ,以RT PCR法间接测定SOCS 3mRNA水平 .结果表明 :a 在左利鼠右侧皮层中 ,SOCS 3基因表达水平显著高于左侧皮层 (P <0 0 5 ) ;右利鼠左侧皮层SOCS 3表达显著高于左利鼠左侧皮层 (P <0 0 5 ) .b 海马中SOCS 3表达 :左利鼠右侧海马SOCS 3表达显著高于左侧海马 (P <0 0 5 ) ;右利鼠左侧海马SOCS 3表达显著高于左利鼠左侧海马 (P <0 0 5 ) .c 右利鼠下丘脑中SOCS 3显著高于左利鼠 (P<0 0 5 ) .上述研究结果提示 ,Balb/c小鼠脑中SOCS

To investigate the expression of suppressor of cytokine signaling 3 (SOCS-3) in Balb/c mice brain and the association with brain lateralization, the paw preference test was used to select right-pawed, left-pawed mice. The SOCS-3 expression level was detected by RT-PCR. The results showed that, I) The SOCS-3 expression level in right cortex was higher than that in, left cortex of left-pawed mice ( P < 0.05 and it was also higher in the left cortex of right-pawed mice than that in the left cortex of left-pawed mice ( P < 0.05), (2) The SOCS-3 expression level in right hippocampus was higher than that in the left hippocampus of left-pawed mice ( P < 0.05) and it was also higher in the left hippocampus of right-pawed mice than that in the left hippocampus of left-pawed mice ( P < 0.05), ( 3) The SOCS-3 expression level in hypothalamus of right-pawed mice was higher than that of the left-pawed mice ( P < 0.05). These results indicate that the SOCS-3 expression level in Balb/c mice brain is associated with brain lateralization.