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咪唑啉-1受体哺乳动物细胞稳定表达系统的建立 被引量：2

Stable expression of imidazoline-1 receptor in mammalian cells
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摘要 目的 :在中国仓鼠卵巢 (CHO)细胞上稳定转染咪唑啉 1受体。方法 :用脂质体介导方法将I1R蛋白cDNA稳定转染到缺乏天然I1R的CHO细胞中。结果 :建立了单独稳定转染I1R而无α2 肾上腺素受体表达的细胞系 ,为独立考察I1R的功能和信号转导通路提供了理想的细胞模型。结论 :成功建立了稳定表达咪唑啉 1受体的细胞模型。 Objective: To stably transfect imidazoline-1 receptor (I 1R) in Chinese hamster ovary (CHO) cells. Methods: I 1R Protein cDNA was stably transfected in CHO cells by the method of lipofectamine. Results: The cell line that stably expressed I 1R without the expression of α 2-adrenergic receptors was constructed. This cell line provided a perfect cell model to investigate the function and signal transduction pathway of I 1R. Conclusion: A cell line with stable expression of I 1R was constructed.
作者 赵琰 苏瑞斌 吴宁 徐波 刘茵 路新强 李锦
机构地区 军事医学科学院毒物药物研究所
出处 《军事医学科学院院刊》 CSCD 北大核心 2004年第4期333-336,共4页 Bulletin of the Academy of Military Medical Sciences
基金 国家重点基础研究发展计划 ("973"计划 )资助项目 ( 2 0 0 3CB5 15 40 0 )
关键词 咪唑啉类 仓鼠属 卵巢 CHO细胞 转染 imidazoles cricetulus ovary CHO cell transfection
分类号 R-332 [医药卫生]
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参考文献9

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同被引文献21

  • 1李斐,吴宁,徐波,赵琰,苏瑞斌,李锦.咪唑啉Ⅰ型受体候选蛋白——非G蛋白偶联受体信号转导与ERK相关[J].军事医学科学院院刊,2005,29(4):325-328. 被引量：1
  • 2Ernsberger P. The I1-imidazoline receptor and its cellular signaling pathways[J]. Ann N Y Acad Sci, 1999, 881: 35-53.
  • 3Piletz JE. Imidazoline receptor antisera-selected(IRAS) cDNA: cloning and characterization[J]. DNA Cell Biol, 2000, 19(6): 319-329.
  • 4Moldering GJ, Moura D, Fink K, et al. Binding of [3H]clonidine to I1-imidazoline sites in bovine adrenal medullary membranes[J]. Naunyn Schmiedebergs Arch Pharmacol, 1993, 348(1): 70-76.
  • 5Davis RJ. Signal transduction by the JNK group of MAP kinases[J]. Cell, 2000,103(2):239-252.
  • 6Gutkind JS. Cell growth control by G protein-coupled receptors: from signal transduction to signal integration[J]. Oncogene, 1998, 17(11):1331-1342.
  • 7Piletz JE, Wang G, Zhu H. Cell signaling by imidazoline-1 receptor candidate, IRAS, and the nischarin homologue[J]. Ann N Y Acad Sci, 2003,1009:392-399.
  • 8Sano H, Liu SC, Lane WS,et al. Insulin receptor substrate 4 associates with the protein IRAS[J]. J Biol Chem, 2002, 277(22): 19439-19447.
  • 9Edwards L, Fishman D, Horowitz P, et al. The I1-imidazoline receptor in PC12 pheochromocytoma cells activates protein kinases C, extracellular signal -regulated kinase (ERK) and c-jun N-terminal kinase(JNK)[J]. J Neurochem, 2001, 79(5): 931-940.
  • 10Zhang J, El-Mas MM, Abdel-Rahman AA.Imidazoline I1 receptor-induced activation of phosphatidylcholine-specific phospholipase C elicits mitogen-activated protein kinase phosphorylation in PC12 cells[J]. Eur J Pharmacol , 2001,415(2-3):117-125.

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军事医学科学院院刊
2004年 第4期

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