补肝养髓方对自发老年性痴呆模型中枢胆碱能系统活性的影响

Effects of Bugan Yangsui Formula on the Activity of Central Cholinergic Nerve System in Spontaneous Aged Dementia Model

目的 观察补肝养髓方对自发老年性痴呆模型学习记忆功能及中枢胆碱能活性的影响。方法 用跳台实验 (step -downtest)从 2 1月龄昆明种小鼠筛选出自发老年性痴呆 (记忆障碍 )鼠 ,随机分为 4组 ,即空白对照组、西药对照组、补肝养髓小剂量组、补肝养髓大剂量组 ,另设老年学习记忆正常组 (老年正常组 )。西药对照组给予喜得镇(Hydergine) 0 .6mg/kg ,补肝养髓小剂量、大剂量组分别予补肝养髓方 6.80 g /kg及 2 0 .41g/kg ,连续 60d ,正常对照和痴呆对照组均灌以等量双蒸馏水(DW )。观察结束后取脑组织冰冻切片 ,Hedreen法示AChE纤维 ,Burt及Silver法示ChAT ,全自动显微图像分析系统定量检测相关脑区AChE纤维及ChAT活性。结果 补肝养髓方能显著增强中枢ChAT活性 ,增高大脑皮质和海马胆碱能纤维密度 ,而且其作用呈现出量效相关关系。结论 补肝养髓方可显著增强其中枢胆碱能系统活性。

Objective To observe the effects of Bugan Yangsui formula on the ability of learning and memory and the activity of central cholinergic system in spontaneous aged dementia mice.Methods Using step-down test,the spontaneous aged dementia mice were screened out from 21 months old Kunming mice and then were divided randomly as 4 groups: control, western medicine, lower dosage Bugan Yangsui formula and higher dosage Bugan Yangsui formula group, in addition, normal aged mice group was also set. Western medicine group was treated with Hydergine with the dosage of 0.6 mg/kg, while Traditional Chinese Medicine was given with the dosage of 6.80 g/kg and 20.41 g/kg respectively in lower dosage and higher dosage Bugan Yangsui formula group, control and normal aged group were treated with equal volume DW. All groups were treated as aforementioned for 60 days. Brain tissue was made to frozen section. Using Hedreen's method to show AChE fiber,using Burt and Silver's method to show ChAT, and using automatic micro-image analysis system to determine quantitatively.Results Bugan Yangsui formula could improve the disturbance of learning and memory in spontaneous aged dementia mice remarkably, could enhance the activity of ChAT in CNS, moreover, could increase the density of cholinergic fibers in cerebra cortex and hippocampus. All these effects had dose-response relationships.Conclusion Bugan Yangsui formula can significantly improve the activity of central cholinergic system in spontaneous aged dementia mice