丹参对脊髓损伤早期NO含量影响的实验研究

Experimental Study of Nitric Oxide on the Early Stay of Spinal Cord Injury Influenced by Salvia Miltiorrhiza

目的 探讨丹参对脊髓损伤 (spinalcordinjury ,SCI)早期防治的作用机制。 方法 采用新西兰大白兔 3 2只 ,随机分为 4组 :对照组 ,损伤组 ,丹参治疗组 ,L -NAME(L -NG-硝基精氨酸甲酯 )组 ,每组 8只。除对照组外 ,其他组均按改良的Allens法致伤造成脊髓损伤模型。各组做相应处理 ,伤后 4h、6h取血液标标 ,6h取脊髓标本 ,测定血液和脊髓组织中一氧化氮 (NO)的浓度 ,并在光镜下观察脊髓的组织形态学的变化。结果 损伤组血液标本和脊髓标本NO升高 ,与对照组比较有统计学意义 (P <0 .0 5 ) ,丹参组血液和脊髓NO含量与对照组和L -NAME组比较无统计学意义 (P >0 .0 5 )。组织学形态上 ,伤后 6h损伤组脊髓出血坏死比较严重 ,大部分白质有损害 ,神经元细胞损害较多 ,而丹参组脊髓出血比较轻 ,大部分白质存留 ,神经元细胞破坏少。结论 脊髓损伤后血液和脊髓组织NO的含量异常升高 ,丹参能降低NO浓度的异常升高 ,减少NO对细胞脂质膜的损害 ,从而保护脊髓 ,在一定程度上阻止继发损伤的进一步发展。

Objective To study the changes of nitrous oxide (NO) in blood and spinal cord after spinal cord injury and the influence of Salvia Miltiorrhiza.Methods 32 rabbits were divided into four groups: normal group, injuried group, Salvia Miltiorrhiza group, N-nitiro-Larginine methylester(L-NAME) group. Spinal cord injury patterns of all groups were made by Allen's method. Except normal group and injury group ,Salvia Miltiorrhiza group and L-NAME group were injected in abdomen with Salvia Miltiorrhiza and L-NAME respectively. The blood and spinal cord was take out at 6 hour. Then the content of NO was tested. And the pathological change of spinal cord was examined.Results After SCI, NO decreased in both blood and spinal cord with Salvia Miltiorrhiza.Conclusion NO in blood and spinal cord were increased after SCI. NO can be decreased at a certain extent with Salvia Miltiorriza .It can prevent secondary injury after SCI.