摘要
目的 研制非典型肺炎病毒 ( SARS病毒 )不同基因区抗原 ,探讨 SARS病毒感染机体不同基因区抗体免疫应答。方法 根据目前已知 SARS病毒的基因组序列及其编码的蛋白质 ,运用计算机抗原表位预测技术 ,筛选 4种 SARS病毒主要抗原表位。合成抗原表位基因 ,克隆到表达载体 p BVIL1 ,在 E.coli中表达 S蛋白和 N-蛋白 ,E-蛋白和 M-蛋白为合成肽。结果 用 SARS病毒编码的 S( Spike)蛋白、M-蛋白、N-蛋白和 E-蛋白分别与 46份 SARS患者恢复血清反应 ,检测血清中 Ig G抗体 ,结果 S蛋白、N-蛋白、E-蛋白和 M-蛋白检出率分别为 71 .7% ( 33/4 6) ,89.1 3% ( 4 1 /4 6) ,30 .43% ( 1 4/4 6) ,5 2 .1 7% ( 2 4 /4 6)。结论 感染 SARS病毒病人血清中 S蛋白、M-蛋白、N-蛋白和
Objective To study the reactivity of recombinant and synthetic antigens derived from different structural genes of SARS-Coronavirus toward convalescent sera of SARS patients (as a probe into the humoral immune response induced by the virus).Methods Multi-epitope chimeric recombinant SARS-CoV 'Spike' protein and 'Nucleocapsid' protein antigens and synthetic E & M peptide antigens were constructed using epitope prediction software to scan the published SARS-CoV genomic sequence.Purified antigens were used to coat microplates and presence of corresponding antibodies in convalescent sera was detected using an indirect ELISA format.Results The number of positive reactive sera over the number of convalescent sera tested using the recombinant S and N antigen of SARS-CoV and E and M peptide antigens were as follows:'S' 33/46(71.7%),'N'41/46(89.13%),'E'14/46(30.43%) and'M'24/46(52.17%).Conclusion There exist quite marked differences among antibody responses in SARS convalescent sera when tested with recombinant or synthetic antigens derived from different structural genes.
出处
《现代检验医学杂志》
CAS
2004年第4期1-3,共3页
Journal of Modern Laboratory Medicine
基金
国家"8 63"科技攻关项目资助 (编号 :43 45 12 63 2 )
关键词
SARS病毒
不同基因区抗原
反应性
SARS-Coronavirus
different genes antigens of SARS-CoV
reactivity