缺血性中风后遗症模型的建立与评价

Establishment and Assessment of Ischemia Stroke Sequela Model

目的:模拟人类中风病过程,界定缺血性中风后遗症大鼠模型。方法:电凝法制成大鼠大脑中动脉闭塞(MCAO)模型,用大鼠走横木试验(BWT)评分法,自MCAO术后3d开始,动态观察大鼠四肢精细运动功能积分;结合同期脑组织病理形态学改变;免疫组化方法观察病灶区及周围反应区生长相关蛋白(GAP-43)、突触素P38(SYN)、细胞骨架蛋白(MAP-2、NF200)、氧化还原因子-1(Ref-1)的表达以及神经细胞凋亡的变化;ELISA法观察血清神经元特异性烯醇化酶(NSE)的动态变化等,研究上述变化的特点及其与病理过程间的关系。结果:神经行为学观察表明大鼠MCAO5周后功能恢复曲线呈现平台势线;病理形态学显示MCAO术后4周病变区形成致密瘢痕组织,5周后病灶区及其周边形成异形胶样变性;免疫组化和生化检测结果提示MCAO术后4周内是模型大鼠进行组织结构修复和功能恢复的时间。结论:大鼠MCAO5周后为缺血性中风后遗症大鼠模型。

Objective:To establish ischemia stroke sequela model of rats so as to similate the process of human stroke. Method To make a MCAO model by the method of electrocaogulation and dynamically observe the functional integral of the fine motors of the rats' limbs according to the criteria of BWT score after 3 days of MCAO, synchronously survey the change of pathomorphology in brain tissues and monitor the expression and the apoptosis of neurocytes of GAP-43, P48 SYN, MAP-2, NF200 and Ref-1 in the zones of focus and peripheral reaction by immunohistochemical method as well as the dynamic change of NSE with serum neuron specificity by means of ELISA, and study the characteristics of the above-mentioned changes and their relations with pathological processes. Result The observation from neuroethology indicates that the recovery of the motor functions of the experimental rats assumes in a curve of platform trend after 5 weeks of MCAO. Their pathomorphology shows that pykno-scar tissues are formed in focus zones after 4 weeks of MCAO and abnormal colloid degeneration also formed in focus zones and their ambience after 5 weeks. The immunohistochemical and biochemical tests suggest that it is the very time for model rats to have their plerosis in tissues and get functionally recovered within 4 weeks after MCAO. Conclusion Ischemia stroke sequela model of rats can be established in 5 weeks after MCAO.