沙尘暴颗粒物对人肺成纤维细胞的细胞毒性研究

Study on the Cytotoxicity of Dust Storm PM_(10) and PM_(2.5) on Human Lung Fibroblasts

目的探讨沙尘暴颗粒物(PM10、PM2.5)对人肺成纤维细胞的细胞毒性。方法分别用50、100、150、200μg/ml的PM10和PM2.5对人肺成纤维细胞进行染毒,24h后测定细胞线粒体琥珀酸脱氢酶活力、细胞外乳酸脱氢酶和酸性磷酸酶的漏出量。结果50μg/ml以上浓度的沙尘暴颗粒物可剂量依赖性地抑制人肺成纤维细胞的线粒体琥珀酸脱氢酶活力。在更高染毒浓度,沙尘暴颗粒物可引起细胞外乳酸脱氢酶和酸性磷酸酶的漏出增加。结论沙尘暴颗粒物对人肺成纤维细胞可产生明显的毒性作用。线粒体可能是沙尘暴颗粒物毒作用的敏感部位。

Objective To investigate the cytotoxicity of dust storm PM10 and PM2.5 on human lung fibroblasts. Methods Human lung fibroblasts were treated with PM10 and PM2.5 at the concentrations of 50, 100, 150 and 200 μg /ml respectively. After treatment for 24 h, the activity of mitochondrial succinate dehydrogenase, the leakages of lactic acid dehydrogenase and acid phosphatase into the culture medium were determined. Results Dust storm PM10 and PM2.5 dose-dependently caused the decrease in the activity of mitochondrial succinate dehydrogenase of human lung fibroblasts at the concentrations above 50 μg/ml. At the higher concentrations, the increase in the leakage of lactic acid dehydrogenase and acid phosphatase was also observed in cells treated with dust storm PM10 and PM2.5. Conclusion Dust storm particles showed cytotoxic effects on human lung fibroblasts. Mitochondria might be the target organelle of dust storm particles.