4-胺基安替吡啉法测挥发酚的标准曲线稳定性分析

Analysis of stability for calibration curve about determination of phenols with method of 4-Amino antipyrine

目的 掌握和控制影响酚标准曲线斜率的因素 ,认识回归方程的截距与空白测定值的关系 ,重新界定标准曲线的线性范围 ,提高酚标准曲线的精密性 ,减少批期内标准曲线测定次数 ,从而方便工作节省成本。方法 用统计学的方法分析酚标准曲线各点测定值及斜率的集中趋势、离散趋势并对酚标准曲线回归方程的截距及空白测定值做配对t检验。结果 控制了实验条件的各条酚标准曲线各点的测定值的标准差小于仪器的检出限 ,斜率趋于平均值 ;按国际法测定酚的线性范围可达 0 .16mg/L。结论 相同条件下 ,国际法测挥发酚时 ,有质控的用一条初始标准曲线进行定量计算即可[1〗;浓度在 0 .16mg/L以下的样品不必稀释可直接测定。

Objective Control slope of the curve and y-intercept,redefine linear scope,promote curve precision,and decrease repeating determination.Methods Analyse central tendency and tendency of dispersion with data of curve and make a matched t-test for data of method blank to y-intercept.Results The result shows a few change of the slope and the linear scope can reach 0.16mg/L.Conclusion In experiment term we can calculate data by one calibration curve controlled with standards and can direct determination samples ranged within 0.16mg/L.