哮喘患者血清嗜酸性粒细胞趋化蛋白测定及其临床诊断价值

Detection of Eotaxin and Its Clinical Diagnosis Value in Patients with Bronchial Asthma

目的探讨嗜酸性粒细胞趋化蛋白(Eotaxin)在哮喘发病中的作用及其临床诊断价值。方法应用ELISA方法测定38例过敏哮喘患者、28例非哮喘过敏体质患者及30例正常对照者血清Eotaxin水平。结果哮喘患者血清Eotaxin水平较非哮喘过敏体质患者及正常对照者显著增高(P<0.01)。哮喘急性发作患者Eotaxin水平较哮喘稳定组患者明显升高(P<0.001);哮喘急性期血清Eotaxin水平与外周血嗜酸粒细胞总数正相关(r=0.4196熏P<0.001)。与1秒钟用力呼气容积(FEV1)负相关(r=-0.3746熏P<0.001)。结论Eotaxin可能通过对过敏性炎性细胞的激活和从血中向支气管粘膜浸润过程的调控,与哮喘的病理生理过程有关,尤其与哮喘急性发作和疾病严重程度密切相关。Eotaxin有可能成为哮喘治疗的新靶点。

Objective To explore the role of eotaxin in the pathogenesis of bronchial asthma and the clinical value in the diagnosis of asthma. Methods Serum eotaxin were measured by ELISA in 38 patients with asthma, 28 patients with non-asthma allergy, and 30 healthy controls. Results The levels of serum eotaxin in the asthma group were higher than those in the non-asthma allergic and control group(P < 0.01). Furthermore, eotaxin levels in patients with acute asthma were significantly higher than those in patients with stable asthma(P < 0.001). It was also found that the eotaxin levels of the acute asthma group were positively correlated to the amounts of eosinophils in peripheral blood(r = 0.4196, P < 0.001),and inversely correlated to the forced expiratory volume in one second(FEV1)(r = -0.3746, P < 0.001). Conclusion It suggests that eotaxin may play a crucial pathogenic role in the asthmatic process possibly by activating the allergic inflammatory cells and controlling the recruitment of eosinophils from blood to bronchial epithelium of the airway. The concentration of eotaxin is significantly associated with the attack of acute asthma and its severity. Eotaxin may be a potential therapeutic target in patients with asthma.