肝素经纤支镜给药的血药浓度监测

Determination of the concentration of heparin in plasma through fiberbronchoscopic administraton of drug

目的 :建立肝素的血药浓度测定方法并对肝素经纤支镜给药的血药浓度进行监测。方法 :以生色底物法进行测定。结果 :肝素在 0 .0 1～ 1 .0 0U·mL-1 范围内线性关系良好。血浆肝素的平均回收率为 97.0 3%,RSD为 1 .82 %(n =6 )。结论 :该方法简便 ,准确 ,适用于血浆中肝素的血药浓度测定。

OBJECTIVE To establish a method for determination of the concentration of heparin in plasma through fiberbronchoscopic administration of drug. METHODS Using the method of chromogenic substrates. RESULTS The linear range for the determination of heparin was 0.01～ 1.00 U·mL -1, the average recovery of heparin in plasma was 97.03%,RSD was 1.82%(n=6). CONCLUSION This method was simple and accurate,and could be used for determination of heparin in plasma.