疣毒净霜剂的研制及其体外药效学

Preparation and in vitro pharmacodynamics effect of the Youdujing cream

目的 :研制治疗尖锐湿疣的疣毒净霜剂 ,并进行其抗人乳头瘤病毒 (HPV)的体外药效研究。方法 :以鸦胆子、紫草、莪术、细辛等为主药 ,制备水包油型霜剂。将霜剂作用于离体尖锐湿疣皮损组织中的HPV ,应用荧光定量聚合酶链反应 (FQ PCR)技术 ,通过定量检测HPV DNA的扩增情况 ,研究其抗病毒作用。结果 :经疣毒净霜剂作用后 ,DNA扩增受到抑制 ,检测结果为阴性 ;而对照组的DNA数则达每 1mL 1× 1 0 7拷贝 ,扩增没有受到抑制 ,结果为阳性。结论 :疣毒净霜剂制备工艺简单、稳定性好 ,具有明显的抗人乳头瘤病毒作用。

OBJECTIVE To prepare the Youdujing cream for the treatment of the condyloma acuminatum (CA),and study its anti-human papillomavirus(HPV) pharmacodynamics in vitro. METHODS The cream was prepared using brucea fruit,zedoary,arnebia and asarum. The cream was acted on the HPV in the tissue of the isolated CA.The polymerase chain reaction (FQ-PCR) was used for the study of antiviral effect by determining the HPV-DNA amplification. RESULTS After using the drug,the HPV-DNA amplification were restrained,the test showed negative, but the reference groups appeared positive,the amplification was not restrained and the number of DNA is 1×107 copy·mL -1.CONCLUSION The Youdujing cream is effective to anti-human papillomavirus.