HPLC法分析五灵胶囊抗肝细胞损伤的药效成分

Efficienct components analysis of WuLing Capsule in hepatic cell lesion by HPLC technique

目的 :分析五灵胶囊抗肝细胞损伤的药效成分及保护作用 .方法 :采用高效液相法 (HPLC) ,将已知的对照品作对照 ,测定五灵胶囊中所含相应成分的含量 ;以体外D GlaN、LPS诱导的大鼠原代肝细胞为靶细胞 ,经细胞培养观察五灵胶囊及对照品对肝细胞损伤后的保护作用 .结果 :在与对照品对照条件下 ,经HPLC分析五灵胶囊中各成分的滞留时间与各对照品时间一致 ;五灵胶囊和其他 3对照品可使D GlaN、LPS诱导损伤原代肝细胞的ALT ,LDH L ,MAO ,GSH ST和MDA水平显著降低 ,有效地拮抗D GlaN和LPS对肝细胞的损伤 ;在受损肝细胞内测定五灵胶囊的化学成分有五味子醇甲、五味子乙素、隐丹参酮和丹参酮ⅡА .结论 :HPLC分析和细胞药效学证实 ,初步推断五灵胶囊抗肝细胞损伤的部分有效成分是乙素、隐丹参酮和丹参酮ⅡА 。

ALM: To investigate the chemical components of WuLing Capsule and its protective effects on the liver cell lesion. METHODS: With the pure monomer component as positive control, we analyzed the components of WuLing Capsule by HPLC. Hepatic cell damage model was prepared by the induction of D GlaN and LPS in vitro separately and then the two different protective effects were compared. RESULTS: Both WuLing Capsule and pure component could obviously depress the levels of ALT, LDH L, MAO and GSH ST, which were higher in damaged primary hepatic cells induced by D galN and LPS. Schisandrin B, Crytotanshinone and TanshinoneⅡA were the efficient components analyzed from nutrient solution and hepatic cells. CONCLUSION: Schisandin B, Crytotanshinone and TanshinoneⅡA are the effective chemical components of WuLing Capsule, which have some protective effects on the liver cell lesion.