成人骨髓间充质干细胞体外诱导向神经细胞分化

Culture and neuronal induction of human bone mesenchymal stem cells in vitro

目的 :探讨成人骨髓间充质干细胞 (MSC)向神经细胞体外定向诱导分化的条件 .方法 :从正常成人志愿者髂骨中分离获取MSCs ,体外培养扩增纯化后传代于塑料培养皿中 ,采用 2 巯基乙醇 (BME)和二甲亚砜 (DMSO)对传至 3～ 5代的细胞进行体外诱导分化 ,采用免疫细胞化学法对诱导后的细胞鉴定 .结果 :诱导 1h有部分细胞表达神经干细胞标志巢蛋白nestin ,4h后大部分细胞具有典型神经元形态 ,表达晚期神经元标志物 中间神经丝蛋白 (NF M) ,部分具有神经元形态的细胞表达微管相关蛋白 2 (MAP 2 ) .结论

AIM: To investigate the conditions for the neuronal induction of bone mesenchymal stem cells (MSCs) from adult human. METHODS: The MSCs were isolated primarily from bone marrow of adult volunteers’ iliac and purified by passage culture. The 3rd to 5th passage of MSCs were planted into plastic petridishes and induced by 2 mercaptoethanol (BME) and dimethyl sulfoxide (DMSO). Immunocytochemistry was used to identify cell types. RESULTS: After a 1 hour induction, some of the MSC derived cells expressed nestin, a neural stem cell marker. After a 4 hour induction, a large number of cells displayed classical neuron like morphology and expressed neurofilament protein M (NF M), and some of the cells exhibiting neuronal morphologies expressed microtube associated protein 2 (MAP 2), which was a neuron marker. CONCLUSION: MSCs from adult human can be cultured, proliferated and differentiated into neuron like cells in vitro .