长期砷诱导L-02细胞的表达谱基因芯片实验

Expression fingerprint analysis of chronic arsenite-induced L-02 cell using cDNA microarry

目的 :采用基因芯片技术研究长期砷染毒时人正常肝细胞 (L 0 2细胞 )基因表达谱的变化 .方法 :4 μmol/LNaAsO2染毒人正常肝细胞 (L 0 2细胞 ) 2wk以上与未染毒的L 0 2细胞做表达谱基因芯片杂交 .结果 :长期砷染毒后细胞基因出现多方面表达差异 ,既包括细胞一些正常生理功能如生长发育、分裂增殖相关的基因 ,同时也包含一些与肿瘤发生、发展有关的基因 .结论 :细胞长期暴露于砷环境后多种与肿瘤发生有关的基因差异表达 。

AIM: To investigate the expression profile of human genes in response to chronic sodium arsenite treatment by cDNA microarrays. METHODS: The RNA was purified from the L 02 cells without and with 4 μmol/L arsenite sodium induction for more than 2 weeks, respectively. RESULTS: In long term exposure, many genes were aberrantly expressed, which included genes involved in cell cycle regulation, signal transduction, stress response and growth factor and various oncogenes. CONCLUSION: Many tumor related genes are aberrantly expressed when cells are exposed to arsenic environment for more than two weeks, which may offer some clues to further studies on carcinogenesis and arsenite.