芪丹通脉片对动脉粥样硬化大鼠血清NO含量及动脉壁eNOS基因表达的影响

Effects of QiDanTongMai Tablets on nitric oxide content in serum and gene expression of endothelium nitric oxide synthase in artery vessels of experimental atherosclerotic rats

目的 :观察芪丹通脉片 (QDTMT)对实验性动脉粥样硬化 (AS)大鼠血清一氧化氮 (NO)含量和动脉壁匀浆中内皮型一氧化氮合酶 (eNOS)基因表达的影响 ,探讨QDTMT的抗AS机制 .方法 :将健康雄性SD大鼠 72只随机分为 6组 ,每组1 2只 :模型组 (model)、空白对照组 (control)、阳性对照辛伐他汀组 (simvastatin)、QDTMT低剂量组 (QDTMT L)、QDTMT中剂量组 (QDTMT M)、QDTMT高剂量组 (QDTMT H) .采用高脂饮食配合灌胃给予维生素D3建立大鼠AS模型 ,各组动物灌胃给药 .采用萘胺重氮分光光度法测定血清NO含量 ,采用半定量RT PCR方法检测各组动物动脉壁匀浆中eNOS基因的表达 ,分析造模及各药物组血清NO含量及eNOS基因表达的变化 .结果 :与空白对照组相比 ,模型组和各用药组血清NO含量均明显增加 (P <0 .0 1 ) ;模型组eNOS基因的表达较空白对照组明显减少 (P <0 .0 1 ) ,与模型组相比 ,辛伐他汀组及各中药组均能增加eNOS基因的表达 (P <0 .0 1 ) ,且QDTMT各剂量组之间存在量效关系 .结论 :QDTMT能增加AS大鼠动脉壁eNOS基因的表达 。

AIM: To investigate the effects of QiDanTongMai Tablets (QDTMT) on the content of nitric oxide in the serum and the gene expression of endothelium nitric oxide synthase in artery vessels of experimental atherosclerotic rats. METHODS: Seventy two healthy male SD rats were divided randomly into 6 groups, with 12 rats in each group: model group, control group, simvastatin group, QDTMT low dosage group (QDTMT L), QDTMT moderate dosage group (QDTMT M) and QDTMT high dosage group (QDTMT H). The rat model of atherosclerosis (AS) was established by feeding high cholesterol diet and vitamin D3. Naphthylamide heavy nitrogen spectrophotography was adopted to detect the content of nitric oxide in the serum and half quantitative reverse transcription polymerase chain reaction (RT PCR) was used to detect eNOS gene expression in artery vessels. The nitric oxide content changes and the gene expression of eNOS in artery vessels of each group were analyzed. RESULTS: The content of nitric oxide in the serum of model and each treated group was significantly higher than that in control group( P <0.01-0.05). The eNOS mRNA expression level in the model group was significantly lower than that in control group( P <0.01), but the eNOS mRNA expression level in each treated group was significantly higher than that in the model group ( P < 0.01- 0.05). CONCLUSION: QDTMT can enhance the gene expression of eNOS in artery vessels of experimental atherosclerotic rats, which may be one of the anti atherosclerosis mechanisms of QDTMT.