大鼠光气染毒后机体氧化损伤及NaHCO_3缓冲液对其的影响

Phosgene-induced oxidative injury in rats and effects of NaHCO_3 buffer on it

目的 :探讨大鼠光气染毒后机体内氧化指标的改变及NaHCO3 缓冲液对其的影响 ,研究光气肺损伤的机制以及中毒的救治方法 .方法 :SD大鼠 4 0只随机分成 4组 .染毒后 ,用两个剂量水平的NaHCO3 缓冲液腹腔注射 ,染毒后 3h断头采血并剖腹取肺和肝脏 ,取肺左叶称取湿干比 ,用荧光法分别检测肺脏、血清和肝脏的脂质过氧化产物丙二醛 (MDA)、还原型谷胱甘肽 (GSH)和氧化型谷胱甘肽 (GSSG) .结果 :单纯染毒组肺脏MDA和血清MDA ,GSH ,GSSG及肝脏的GSSG与对照组比较 ,差异显著 ;0 .5mLNaHCO3 缓冲液治疗组肺脏MDA和血清MDA ,GSH与单纯染毒组相比差异显著 ,0 .2mLNaHCO3 缓冲液治疗组血清GSH和肝脏GSSG与单纯染毒组比较 ,差异显著 .结论 :光气染毒后机体氧化损伤 ,给予NaHCO3

AIM: To probe into phosgene induced oxidative injury in rats and the effects of NaHCO 3 buffer on it and to investigate the mechanism of pulmonary edema formation induced by phosgene and the therapy for phosgene poisoning. METHODS: Forty animals were randomly divided into four groups: control group, phosgene group, 0.2 mL NaHCO 3 buffer group and 0.5 mL NaHCO 3 buffer group. The phosgene, 0.2 and 0.5 mL groups were exposed to phosgene air (11.9 g/m 3×3 min ) and were injected with NaHCO 3 buffer intraperitoneally at the fifth minute. The third hour after exposure, all rats were killed by decollation. Fluorescence was employed to measure MDA, GSH and GSSG of the right lung, serum and liver and the left lung wet to dry weight ratio was calculated. RESULTS: The difference in the lung MDA, serum MDA, GSH and GSSG, and liver GSSG were significant between the control group and phosgene group. Compared with those of phosgene group, the lung MDA and the serum GSSG and MDA of 0.5 mL NaHCO 3 buffer group were significantly different. The serum GSH and liver GSSG of 0.2 mL NaHCO 3 were also significantly different compared with those of phosgene group. CONCLUSION: NaHCO 3 buffer may have relieving effects on oxidative system damage induced by phosgene exposure.