非均匀电场对土壤中基因工程菌的迁移与机理

The movement and its mechanism of genetic engineering bacteria in soils by non-uniform electric field.

以克隆有绿色荧光蛋白基因的Rm1021-GFP细菌作为标记细菌,研究了该细菌在非均匀电场作用下在土壤中的运移特征和存活性,分析了其运移机理和影响因素.结果表明,非均匀电场能有效地促进土壤中Rm1021-GFP细菌的运移,电泳、介电泳和随电渗析流迁移是其主要机理,土壤介质、电压梯度和电极反应是其主要影响因素,电极反应对电极附近细菌的存活性有不利影响.

With Rm1021-GFP bacteria of green fluorescent protein gene as a mark bacterium, the movement character and survival of the bacteria in soil under non-uniform electric field were studied, and the movement mechanisms and affecting factors were analyzed. Non-uniform electric field could promote effectively the movement of the bacteria in soil. Electrophoresis, dielectrophoresis and electroosmosis were its main mechanism; and soil media, voltage gradient and electrolysis of pore fluids on electrodes were its main affecting factors. Electrolysis of pore fluids on electrodes affected adversely the survival of the bacteria near the electrodes.