摘要
研究了一株从活性污泥中分离得到的多食鞘氨醇杆菌(Sphingobacteriummultivorum)对五氯酚(PCP)的共代谢降解.比较了细菌分别以苯酚和葡萄糖为生长底物时对PCP的降解效果.结果表明,苯酚对细菌的生长有一定的抑制作用;葡萄糖支持细菌共代谢降解PCP,葡萄糖和非生长底物PCP之间不存在底物竞争抑制现象.蛋白质电泳结果表明,葡萄糖为生长底物时,PCP的降解酶是由PCP自身所诱导;GC-MS分析PCP共代谢降解中间产物时检测到苯酚的存在,说明在好氧降解情况下,PCP的共代谢降解可能也存在渐次脱氯生成苯酚并最终被矿化的过程.
Cometabolic degradation of pentachlorophenol (PCP) by Sphingobacterium multivorum isolated from activated sludge was studied. The effect of PCP degradation with phenol or glucose as growth substrate was compared. Phenol inhibited definitely the growth of the bacteria and glucose was more suitable for supporting the PCP cometabolic degradation because there was no competitive inhibition between the glucose and the non-growth substrate PCP. Protein electrophoresis results showed that when glucose was as growth substrate, the PCP degradation enzyme was induced by PCP itself. In gas chromatograph-mass (GC-MS) analysis of the intermediate products in PCP cometabolism, the phenol was detected, indicating that even under the condition of aerobic biodegradation, the dechlorination reaction gradually happened and the PCP was mineralized.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2004年第3期294-298,共5页
China Environmental Science
基金
国家高技术研究发展计划资助项目(2002AA649200)
关键词
共代谢
五氯酚
苯酚
葡萄糖
多食鞘氨醇杆菌
cometabolism
pentachlorophenol
phenol
glucose
Sphingobacterium multivorum
