TNF-α对离体培养的少突胶质细胞和髓鞘生长的影响

The effects of TNF-α on the development of oligodendrocyte and myelination and the intervention of Trental in vitro

目的 探讨肿瘤坏死因子 (tumornecrosisfactor α ,TNF α)对离体培养的少突胶质细胞及髓鞘的病理作用及己酮可可碱 (Trental)抗TNF α的作用靶点。方法 采用体外器官型组织培养Wistar大鼠小脑组织及免疫细胞化学方法 ,观察髓鞘生长及表达 2′3′ 环核苷酸 3′ 磷酸二酯酶 (2′3′ cyclicnucleotide,3′ phosphodiesterase,CNPase)阳性的少突胶质细胞数。结果 TNF α及TNF α +Trental组髓鞘形成率明显低于正常对照组 (P <0 .0 5 ) ,TNF α和TNF α +Trental组CNPase阳性细胞数明显较对照组少 (P <0 .0 1 )。结论 TNF α在体外能抑制少突胶质细胞及髓鞘的生长 。

Objective It has been verified that cytokines play an important role in the immunological pathogenetic mechanisms of experimental allergic encephalomyelitis (EAE) and multiple sclerosis (MS).We tried to know the effects of Tumor necrosis factor α(TNF α) on myelination and the development of oligodendrocyte directly in vitro and the target that Trental antagonizes TNF α.Methods By the organotypic culture of the cerebellum of wistar rats and immunocytochemistry,we studied the rate of myelination and counted the amount of 2′3′ cyclic nucleotide,3′ phosphodiesterase(CNPase) positive oligodendrocytes.Results (1) The rate of myelination in TNF α group was significantly suppressed vs control group( P <0.05).(2)The rate of myelination in TNF α +Trental group was significantly inhibited too ( P <0.05,compared with control group).(3) The amount of CNPase positive oligodendrocytes in TNF α and TNF α+Trental group were reduced than control group( P <0.01).Conclusions (1) TNF α can inhibit the development of oligodendrocyte and myelination in vitro.(2) Trental has a negative effect on remyelination in vitro.