原发性肝癌雄激素受体表达的临床病理研究

Clinicopathological study on expression of androgen receptor in hepatocellular carcinoma

目的 探讨原发性肝癌患者雄激素受体的改变对肝癌发生发展的影响。方法 应用放射配体结合分析法(RBA)对 36例行手术切除的肝癌组织、癌周组织及外周血白细胞雄激素受体 (Androgenreceptor,AR)的含量进行测定 ,同时用放射免疫法 (RIA)测定肝癌患者及正常人血浆雄激素 (睾酮T)的水平 ,结合临床病理资料进行统计分析。结果 (1)癌组织与癌周组织相比 ,AR含量明显升高 (P <0 .0 1) ,且与肿瘤直径大小有关 ;(2 )高分化的肝癌组织AR含量明显高于低分化者 (P<0 .0 1) ;(3)肝癌患者血浆睾酮水平增加不明显 (P >0 .0 5 ) ,但白细胞AR表达明显升高 (P <0 .0 1)。结论 肝癌的发生可能与癌组织中AR含量升高有关 。

Objective To study the changes in levels of androgen receptor ( AR) in patients with hepatocellular carcinoma.Methods Radioligand binding assay (RBA) was adopted to measure the androgen receptor ( AR) in cancer tissue and adjacent non-tumor tissue and peripheral blood leucocytes in 36 patients with hepatocellular carcinoma.Hormone radiloimmunoassay(RIA) was performed to determine serum testosterone level in patients and normal subjects.Results (1) The content of AR in the tumor samples was significantly higher than that in the surrounding non-tumor liver tissue(P<0.01),and had a relationship with the size of the tumor; (2)The content of AR in the high differentiated phenotype was higher than that in the less differentiated phenotype(P<0.01); (3)The level of serum testosterone in patients was slightly elevated (P>0.05),but the content of AR in leucocytes was significantly increased(P<0.01).Conclusion The occurrence of hepatocellula carcinoma is probably correlated with higher content of AR,whereas the content of AR is related to the size and differentiation degree of the tumor