CD21非依赖性EB病毒对人胃印戒细胞癌细胞系的感染

CD21-independent infection of a human signet ring cell gastric carcinoma cell line by Epstein-Barr virus

目的 探讨CD2 1非依赖性EB病毒 (EBV)对人胃印戒细胞癌细胞系 (HSC 39)的感染作用。方法 用Akata和P3HR 1EBV毒株感染HSC 39,有限稀释法对感染细胞进行克隆。结果 两种EBV毒株感染细胞中均可检测到EBV编码的小RNA(EBER)的表达 ,两种EBV毒株感染的亲代细胞及大多数细胞克隆表达EBV核抗原 (EBNA1) ,但不表达EBNA2、潜伏期膜蛋白 (LMP1)和LMP2A。表现为潜伏Ⅰ型感染。未感染的HSC 39细胞及P3HR 1感染的细胞克隆CD2 1表达阴性 ,而AkataEBV感染的部分细胞克隆CD2 1mRNA阳性。结论 EBV可能通过不依赖CD2 1受体的途径感染HSC 39,印戒细胞癌细胞系可用作EBV感染的靶细胞。

Objective To understand Epstein-Barr virus (EBV) infection of gastric carcinoma cells. Methods The authors tested the infection of a signet ring cell line HSC-39 derived from human gastric carcinoma with Akata and P3HR-1 strains of EBV. Akata and P3HR-1 infected of EBV cell clones were isolated by a limiting dilution method. Results EBV-encoded small RNAs (EBERs) were expressed in the infected cells with each EBV strain by in situ hybridization. The EBV infected parental cells and most clones expressed EBNA1,but not EBNA2,latent membrane protein (LMP) 1 and LMP2A. Both EBV strains infected parental cells and clones presented typeⅠlatency. The uninfected HSC-39 cells were negative for CD21 expression; however,the Akata but not P3HR-1-infected clones were positive for CD21 expression at mRNA level.Conclusion These results demonstrated that EBV infecting HSC-39 by CD21-independent pathway.This study also defined a signet ring cell line as a new target for EBV.