嗜碱性芽孢杆菌产高碱碱性蛋白酶发酵培养基及发酵条件的研究

The Study on the Medium and Conditions of Bacillus alcalophilus Producing the High Alkaline Protease

对一株高产高碱碱性蛋白酶的嗜碱性芽孢杆菌的发酵培养基及发酵工艺进行了优化。确定了发酵培养基所采用的棉籽饼粉最适粒度为 80目 ,麦芽糊精的最佳DE值为 3 0 %。并确定了该菌株的最适发酵培养基配方为 ( g/1 0 0mL) :棉籽饼粉 3 ,酵母浸粉 1 75 ,麦芽糊精 1 0 ,柠檬酸钠 0 3 ,CaCl2 0 3。K2 HPO4 1 ;最适摇瓶发酵条件为 :种龄 1 2h ,接种量 2 %,装液量 5 0mL/2 5 0mL ,摇床转速 2 0 0r/min ,3 4℃ ,发酵 5 4h ,碱性蛋白酶的发酵单位可达 3 3 985u/mL ,比优化前提高了5 4 48%。此外 ,还进行了 5L罐放大实验 ,在 5L罐所确定的最适工艺条件下 ,发酵单位可达3 65 79u/mL。

The fermentation medium and conditions of Bacillus alcalophilus, which produces high-yield alkaline protease,were studied in this paper The optimum granularity of cotton seed meal was 80mu and the optimum DE of maltrin was 30% The highest enzyme activity was obtained using 50?mL of culture medium in 250?mL shake flasks at 34℃ and 200 r/min for 54h. The fermentation mediun consisted of (per 100?mL)∶3g cotton seed meal,1 75g yeast extract,10g maltrin,0 3g NaCitrate,0 3?g CaCl 2,1g K 2HPO 4 The optimal inoc2酰欤酰? volume was 2%(v/v) Under optimal conditions,the yield of alkaline protease is 33 985u/mL, which is 54 48% higher than that of before. The experiment was also carried out in a 5?L fermentor. Under the optimal conditions,the alkaline protease activity reached 36?579?u/mL in the 5 L fermentor.