葡多酚对N-亚硝基化合物诱发的肝细胞SSTR-2 mRNA表达的抑制

Inhibition of grape procyanidin on the expression of SSTR-2 mRNA in the hepatic cells induced by N-nitroso compounds

目的 研究葡多酚 (GPC)对N 亚硝基化合物诱发的大鼠肝细胞生长抑素受体 - 2mRNA(SSTR 2mRNA)表达的影响作用。方法 将大鼠经口灌胃 0 5 %的亚硝酸钠诱发肝细胞突变 ,2个GPC实验组同时经口给予GPC 1 0 0mg kg和 1 0mg kg,用原位杂交技术检测肝细胞SSTR 2mRNA的表达水平。结果 诱变损伤组和高GPC组SSTR 2mRNA表达的阳性细胞率分别为 1 9 89%和 7 83% ,两组之间的差别有显著性意义 (P<0 0 5 )。结论 葡多酚对N 亚硝基化合物诱发的大鼠肝细胞SSTR

Objective To study the effect of grape procyanidin(GPC) on the expression of somatostatin receptor 2 mRNA induced by N nitroso compounds in the hepatic cells of rats.Methods NaNO 2 was fed to Wistar rats to induce hepatocytes′ mutation,and GPC was given in dose of 100mg/kg and 10mg/kg respectively through mouths to the rats of the two experimental groups at the same time. After eight weeks, situ hybridization was applied to measure the expression level of SSTR 2 mRNA in rats′hepatic cells. Results The positive cells rate of SSTR 2 mRNA expressed in the injury comparison group and the high dose GPC group were 19 89% and 7 83% respectively and the difference between the two groups had statistical significance( P <0 05).Conclusion GPC had inhibitory effect on N nitroso compounds induced abnormal expression of SSTR 2 mRNA in hepatic cells of rats.