离子色谱法测定化妆品中巯基乙酸的研究

Study on analysis of thioglycolic acid in cosmetics by ion chromatography

目的 建立离子交换—抑制电导检测色谱法分离测定化妆品中的巯基乙酸。方法 采用高容量阴离子色谱柱IonPac AS1 1 HC ,以 2 5mmol LNaOH +1 %甲醇为淋洗液 ,流速 0 85ml min。样品经三氯甲烷去除部分有机物后 ,进样分析。结果 方法的线性范围广 ,精密度和准确度高 ,相对标准偏差RSD(% ) <0 94 1 ,样品平均加标回收率 91 5 %～ 1 1 0 0 % ,检出限为 2 3mg kg。结论 样品的酸度、共存离子及淋洗液中甲醇对测定无影响。本方法简便、准确可靠 。

Objective An analyticail method of determinating thioglycolic acid in cosmetics by ion exchange chromatography suppressed conductivity detection has been developed.Methods A high capacity anion exchange column, IonPacAS11 HC column was used. 25mmol/L NaOH+1% methanol was used as eluent, and the flow rate was set up at 0 85ml/min.Samples were pretreated by extraction with CHCl 3 to remove some organic substance, and then upper clean liquid was injected.Results The influence of samples' pH, coexist anion and the concentration of methanol in eluent on determination was discussed. The method enjoyed a wide linear range and good precision. The detection limit was 2 3mg/kg. The average recoveries were 91 5%-10 0%, and the relative standard deviation (RSD%) was less 0 941 Conclusion The results showed that the method was simple, accurate and might be good for application.