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转基因作物标记蛋白潮霉素磷酸转移酶活性的测定方法 被引量：2

Methods of hygromycin B phosphotransferase activity assay in transgenic plant
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摘要 潮霉素磷酸转移酶 (HPT)是转基因作物中重要的标记蛋白 ,其活性的测定对含有该标记基因的转基因作物的植株构建、基因沉默、标记基因去除及安全性分析等具有重要的作用。本文综述了潮霉素磷酸转移酶活性测定的几种方法 ,包括放射化学法、抑菌生长法及酶耦联法 ,其中放射化学法用于检测组织粗提蛋白中的HPT活性 。 Hygromycin B phosphotransferase (HPT) is a widely used selectable marker protein of transgenic plant. Detection of its activity is critical to studies on the development of various transgenic plants, silence of inserted gene, marker free system development and safety assessment of transgenic food. In this paper, several methods for detecting the activity of this enzyme were reviewed.
作者 卓勤 杨晓光
机构地区 中国疾病预防控制中心营养与食品安全所
出处 《卫生研究》 CAS CSCD 北大核心 2004年第4期502-504,共3页 Journal of Hygiene Research
基金 国家科技部 973计划 (No .2 0 0 1CB1 0 90 0 7) 国家科技部863计划 (No .AA2 2 1 2 2 91 ) 科技部国家转基因植物研究与产业化专项(No .J0 0 -C - 0 0 3)
关键词 潮霉素磷酸转移酶 转基因作物 标记基因 活性测定 hygromycin B phosphotransferase, transgenic plant, marker gene, activity assay
分类号 Q78 [生物学—分子生物学] Q555 [生物学—生物化学]
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参考文献15

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同被引文献34

  • 1孙星学,柳参奎.水稻可溶性糖蛋白的纯化与鉴定研究[J].植物研究,2006,26(2):187-192. 被引量：3
  • 2汪滢,陈李萍,陈晓,章秀,于晶,王全喜.发菜中超氧化物歧化酶基因的克隆及在大肠杆菌中的表达[J].植物研究,2007,27(3):289-292. 被引量：12
  • 3贾士荣.转基因植物食品中标记基因的安全性评价[J].中国农业科学,1997,30(2):1-15. 被引量：139
  • 4Tian L N. Genetic transformation of Prunus domestica L. u- sing the hpt gene coding for hygromycin resistance as the selectable marker [ J ]. Scientia Horticulturae, 2009, 119 (3) :339 - 343.
  • 5Florea S. Elimination of marker genes from transformed fila- mentous fungi by unselected transient transfection with a Cre-expressing plasmid [ J ]. Fungal Genetics and Biology, 2009,46(10) :721 - 730.
  • 6Malik V S, M K Saroha. Marker Gene Controversy in Trans- genic Plants [ J ]. J of Plant Biochemistry and Biotechnolo- gy,1999,8(1) :1 -13.
  • 7Ballester A, M Cervera, L Pena Evaluation of selection strategies alternative to npt Ⅱ in genetic transformation of citrus[J]. Plant cell reports,2008,27(6) :1005 - 1015.
  • 8Davis B D. The lethal action of aminoglycoside [ J ]. Journal of Antimicrobial Chemotherapy, 1988,22 ( 1 ) : 1 - 3.
  • 9Gritz L J. Davies Plasmid-encoded hygromycin B resist- ance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomy- ces cerevisiae [ J ]. Gene, 1983,25 (2 - 3 ) : 179 - 88.
  • 10Zalacain M, J M Pardo, A JimeNez. Purification and charac- terization of a hygromycin B phosphotransferase from Strep- tomyces hygroscopicus [ J ]. European Journal of Biochem, 1987,162(2) :419-422.

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卫生研究
2004年 第4期

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