针刺对肥胖大鼠下丘脑视上核的作用

Effect of acupuncture on hypothalamic supraoptic nucleus in obese rats

目的 :探讨针刺减肥的作用机理。方法 :采用神经电生理和生化技术观察针刺治疗前后肥胖大鼠的变化。结果 :肥胖大鼠体重、Lee s指数、体脂、空腹血糖 (FBS)、空腹胰岛素 (FINS)和下丘脑视上核 (SON)神经细胞自发放电频率均明显高于正常大鼠 ,而胰岛素敏感性指数 (IAI)水平明显低于正常大鼠。相关分析显示 ,SON神经细胞自发放电频率与肥胖指标、FBS、FINS水平呈正相关 ,而与IAI水平呈负相关。针刺治疗后肥胖大鼠肥胖指标、FBS、FINS和SON神经细胞自发放电频率均明显回降 ,而IAI水平明显回升。结论 :生物机体SON神经功能亢奋可能是产生肥胖重要因素之一。针刺对肥胖机体下丘脑SON神经功能的良性调整作用可能是针刺减肥中枢作用机制之一。

Objective To explore the effect of acupuncture on the nervous function of hypothalamic supraoptic nucleus (SON) in obese rats. Methods Changes of obese rats before and after acupuncture were investigated by neuro-electrophysiological and biochemical techniques and compared with the control group. Results Levels of the body weight, Lees index, body fat, fasting blood sugar (FBS), fasting insulin (FINS) and neuronal spontaneous discharges in the SON were all significantly higher than those in normal rats, while the insulin active index (IAI) was significantly lower. The frequency of neuronal spontaneous discharge in SON was positively correlated with obesity index, FBS and FINS, but was negatively correlated with IAI level. After acupuncture treatment obesity index, FBS, FINS and neuronal spontaneous discharges in SON in the obese rats were significantly lowered, while the IAI level significantly elevated. Conclusion The abnormal excitation of SON may be one of important factors for obesity, and beneficially regulative action of acupuncture on nervous function of SON in the obese organism is possibly one of central mechanisms of acupuncture slimming treatment.