摘要
目的 观察脱氢表雄酮 (DHEA)及其衍生物 7 羰基 脱氢表雄酮 (7 oxo DHEA)对谷氨酸诱导的海马神经损伤的保护作用。方法 原代培养的SD大鼠海马神经细胞 ,MTT比色法检测细胞存活率 ;激光扫描共聚焦显微镜检测细胞内Ca2 + 及自由基随时间变化曲线 ;比色法测定细胞内谷胱甘肽(GSH)水平。结果 DHEA(0 1μmol·L-1)及 7 oxo DHEA(0 1μmol·L-1)预保护后的谷氨酸损伤海马神经细胞胞内Ca2 + 及自由基变化曲线均明显低于无预保护组 ,GSH水平明显高于无预保护组 (P <0 0 1)。结论 DHEA及 7 oxo DHEA对谷氨酸诱导海马神经细胞损伤有保护作用。
Aim To study Dehydroepiandrosterone (DHEA) and 7-oxo-dehydroepiandrosterone (7-oxo-DHEA) protected hippocampal neurons against neurotoxicity induced by glutamate(Glu). Methods Cell survival rate was analyzed using MTT colorimetry, the change of Ca 2+ levels and the levels of free radical in cultured hippocampal neurons were analyzed by the laser scanning confocal microscope, and cellular GSH level was also analyzed. Results Exposure of cultured rat hippocampal neurons to Glu resulted in accumulation of celluar Ca 2+ and cellular free radical were prevented by DHEA (0.1 μmol·L -1 )and 7-oxo-DHEA(0.1 μmol·L -1),and cellular GSH was increased. Conclusion DHEA and 7-oxo-DHEA protected hippocampal neurons against neurotoxicity induced by Glu.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2004年第7期750-753,共4页
Chinese Pharmacological Bulletin
基金
国家"973"重点基础研究发展规划资助项目
NoG2 0 0 0 0 5 70 0 8
