脂多糖联合卡介苗诱发大鼠慢性支气管炎模型的构建

Establishment of chronic bronchitis rat model by combined utilization of Bacillus Calmette-Guerin (BCG) and lipopolysaccharide (LPS)

目的 评价脂多糖联合卡介苗诱发大鼠慢性支气管炎模型的可行性。方法 按照正交设计实验方案 ,在尾静脉注射BCG的基础上 ,气管内注入LPS或生理盐水 ,饲养 3wk或 4wk后 ,取大鼠支气管肺泡灌洗液瑞氏染色后测定中性粒细胞百分数 ,同时利用光镜结合医用图像分析技术观察大鼠支气管肺组织病理形态学变化。结果 尾静脉注射BCG(5mg)和气管内注入LPS(2 0 0 μg)联合应用 ,饲养 3wk后可出现慢性支气管炎的典型病理形态学改变。结论 利用尾静脉注射BCG结合气管内注入LPS的方法制作大鼠慢支模型是用于研究慢性支气管炎发生及发展机制的简便可靠的模型。

Aim To evaluate the respective and combining beneficial effect of Bacillus Calmette-Guerin (BCG) and lipopolysaccharide (LPS) in the initiation of rat chronic bronchitis model. Methods Experiments were performed in 18 young male Sprague-Dawley rats according to orthogonal design. BCG and LPS were injected through caudal vein and endotracheal intubation respectively. After breeding for 3 or 4 weeks, the number of leucocyte and the ratio of neutrophil to leucocyte in bronchoalveolar lavage fluid (BALF) were observed, while the lung tissue specimens which were stained with hematoxylin and eosin (HE) were studied by quantitative method through microscope and image analysis technique. Results Through combining utilization of BCG(5 mg, injected through caudal vein) and LPS (200 μg, injected through endotracheal intubation), the typical morphologic changes of chronic bronchitis were induced after 3 weeks. Conclusion The chronic bronchitis rat model established by combining utilization of BCG and LPS is convenient and simple for the longitudinal study on the mechanism of initiation and progress of chronic bronchitis.