阿霉素纳米粒对白血病多药耐药细胞株K562/ADR耐药性的逆转作用

Reversal effect of adriamycin-loaded nanoparticles on human leukemia multidrug -resisitant cell line K562/ADR

目的 :研究阿霉素纳米粒对人白血病多药耐药细胞株K5 6 2 /ADR耐药性的逆转作用。方法 :采用MTT法测定药物的体外杀伤作用 ,应用流式细胞术测定细胞内药物浓度。结果 :阿霉素纳米粒和阿霉素对K5 6 2细胞细胞毒作用相似 ,K5 6 2 /ADR对阿霉素纳米粒较阿霉素敏感 2 .6 3倍 ,细胞内阿霉素浓度显著增加可能是关键因素。结论

OBJECTIVE To investigate reversal effect on human leukemia multidrug-resistant cell line K562/ADR by adriamycin-loaded nanoparticles.METHODS The reversal effect of the drugs on multidrug-resistant cell line were determin ed by MTT method.Intracellular accumulation of ADR was studied by flow cytometr y.RESULTS The cytotoxicity of adriamycin-loaded nanoparticles and free adriamycin was i dentical in K562 cells,but the chemosensitivity of K562/ADR cells to adriamycin -loaded nanoparticles was increased to 2.63 times compared with that of K56 2/ADR cells to free adriamycin.The increased sensitivity of K562/ADR cells to ad riamycin-loaded nanoparticles was due to a remarkable increasing intracellular accumulation of adriamycin.CONCLUSION Adriamycin-loaded nanoparticles reverse MDR effectively by increasing intracell ular accumulation of adriamycin in K562/ADR cells.