葛根素对红细胞膜脂质过氧化损伤的防护作用

Protection effect of puerarin against lipid peroxidation damage of erythrocytem embrane

目的 :研究葛根素对红细胞膜脂质过氧化损伤的防护作用。方法 :采用 3种活性氧产生体系诱发脂质过氧化为实验模型 ,观察了葛根素对膜脂质过氧化作用的防护作用。结果 :葛根素对黄嘌呤 黄嘌呤氧化酶 (Xan Xo)系统、H2 O2 及UV照射 3种方法引起的细胞膜脂质过氧化产物丙二醛 (MDA)的生成增加均有抑制作用 ,其作用呈剂量依赖性 ,IC50 分别为 16 .95 ,4 .4 4和 14 .90mg·L-1。结论 :葛根素对自由基引起的细胞膜脂质过氧化损伤有防护作用。

OBJECTIVE To investigate the effect of Puerarin on lipid peroxidation damage of erythrocyt e membrane induced by radicals.METHODS The lipid peroxidation of erythrocyte membrane induced by radicals derived from free radicals generation systems models was measured by thiobarbituric acid spec trophotometry.RESULTS Puerarin dose -dependently inhibited the increases of thiobabituric acid reacti ve substance (TBARS) content in the membrane exposed to xanthine-xanthine o xidase system,H 2O 2 or UV light.The IC 50 were 16.95, 11.44, 14. 90 mg·L -1,respectively.CONCLUSION puerarin may protect erythrocyte membrane from the lipid peroxidative damage ind uced by radicals.