白念珠菌菌丝相与酵母相ERG11基因部分序列的比较研究

Comparative Study on Partial ERG11 Gene Sequence of Candida Albicans Between Yeast-Form and Hyphal-Form

目的 通过对白念珠菌菌丝相与酵母相ERG11基因部分序列的比较,探讨两相在DNA水平的差异。方法 对5株分离自同一HIV阳性患者的白念珠菌分别进行菌丝相与酵母相培养后,抽提基因组DNA,PCR扩增ERG11基因(包括部分上游和下游非编码区),用下游引物对部分ERG11基因进行测序。结果CA-7菌丝相与酵母相ERG11基因序列存在差异,分别位于第1547,1587和1617位点。结论 白念球菌菌丝相与酵母相在DNA水平存在差异,在进行白念珠菌的病原真菌学研究中菌丝相为适宜的研究对象。

Objective To investigate the differences on gene sequence between yeast-form and hyphal-form Candida albicans,a comparative study on partial ERG11 gene sequence was conducted. Methods 5 strains C. albicans from a single HIV-infected patient were grown to form yeast cells and hyphal cells respectively, and the genomic DNA was extracted. Then ERG11 was amplified by PCR and sequenced. Results Differences on ERG11 gene sequence between yeast-form and hyphal-form C. albicans were identified in strain CA-7,which were observed respectively at position 1 547, 1 587 and 1 617 of ERG11. Conclusion There were some differences on DNA sequence between yeast-form and hyphal-form C. albicans. Hyphal-form C. albicans was thought to be the appropriate object of medical mycology.