一个新的基因转录本的克隆及序列分析

Cloning and sequence analysis of a novel gene transcript

目的 :筛选与精子发生和 (或 )睾丸发育相关的基因。方法 :将不同发育阶段 (胚胎和成人 )的睾丸组织cDNA探针与人睾丸cDNA芯片进行杂交 ,通过杂交信号的比较 ,筛选出差异表达的基因。结果与结论 :经测序发现该基因全长 13 16bp ,包含1个 10 5 6bp的开放阅读框 ,编码 3 5 1个氨基酸。生物信息学序列分析表明 ,它是p44S10基因家族的一条新的转录本基因 ,该基因的氨基酸序列编码一 2 6S蛋白酶体调控亚基 ,在进化中高度保守 。

Objective To identify genes involved in spermatogenesis and testicular development.Methods Hybridization of human adult and fetal testes cDNA samples was performed with a human cDNA microarray.Results and Conclusions By analysing the hybridization signals,a gene with a differential expression in adult and fetal testis was identified.The full length cDNA of 1?316?bp contained an open reading frame of 1?056?bp which encoded a putative protein of 351 amino acids.Further sequence analysis showed that it was a splicing variant of p44S10 gene family. The deduced protein encoding a regulatory particle of 26S proteasome has been highly conserved during evolution,suggesting the importance of this gene transcript in organism.