苍白球腹侧核毁损对帕金森大鼠旋转行为及纹状体一氧化氮合酶阳性神经元表达的影响

Effects of lesion of ventral pallidum on the rotation behaviour and nitric oxide synthase positive neurons in striatum in rats with Parkinson's disease

目的 :探讨帕金森病 (Parkinson’sdisease ,PD)大鼠模型苍白球腹侧核 (ventralpallidum ,VP)毁损后对旋转行为变化及对纹状体区一氧化氮合酶 (nitricoxidesynthase ,NOS)阳性神经元表达的影响。方法 :采用 6 羟基多巴 ( 6 hydroxydopamine ,6 OH DA)毁损左侧黑质致密部 (substantianigra ,parscompacta ,SNc)和中脑腹侧被盖 (ventraltegmentalarea ,VTA)以建立PD大鼠模型 ,采用微电极毁损其VP ,观察实验动物旋转行为变化 ,并用NADPH黄递酶 (reducedformofnicotinamide edeninedinucleotidephos phatediaphorase ,NADPH d)组织化学染色显示NOS阳性神经元的表达。结果 :VP毁损后PD大鼠旋转行为明显改善 (P <0 .0 5 ) ,纹状体区NOS阳性神经元数量亦有增加 (P <0 .0 5 )。结论 :VP毁损后 ,PD大鼠旋转行为的减少可能与VP异常放电受抑制有关 ,同时NO的增加又加重了对VP的毒性损伤 。

Objective To observe rotation behaviour and nitric oxide synthase positive neurons in striatum of Parkinson's disease (PD) rats affected by lesion of ventral pallidum (VP). Methods The modle of PD was founded by injecting 6?hydroxydopamine (6?OHDA) into the left substantia nigra, pars compacta (SNc) and ventral tegmental area (VTA) of rats. After damaging VP of these rats, their rotation behaviours were observed, then the reduced form of nicotinamide-edenine dinucleotide phosphate diaphorase (NADPH?d) histochemistry staining method was used to observe the change of striatum NOS positive neurons. Result After VP was impaired, the rotating behaviour induced by apomorphin (APO) was improved distinctly (P<0.05), and the NADPH?d histochemistry staining showed the numbers of NOS positive neurons significantly increased(P<0.05). Conclusion After the impairment of VP, the reduced rotation behaviour of the rats probably results from its inhibited abnormal discharge, and the increased NO content in the striatum reinforces the impairment of VP, which in turn enhances the inhibition of abnormal discharge.