糖尿病大鼠椎间盘中白介素1-α和肿瘤坏死因子-α的含量变化

Changes of the interleukin-1α and tumor necrosis factor-α in the lumbar discs of the diabetic rats

目的 :测定糖尿病大鼠椎间盘组织中白介素 1α (IL 1α)和肿瘤坏死因子 α (TNF α)的含量变化 ,并对其意义进行探讨。方法 :3 6只SD大鼠随机分为 2组 ,每组 18只 ,处理组用腹腔单次注射链脲佐菌素溶液 (strep tozotocin ,STZ)造成糖尿病。分别在第 1、 3、 4个月时 ,采用免疫酶联法 (ELISA法 )测定椎间盘组织中IL 1α和TNF α。结果 :在 3个不同时期 ,糖尿病组中IL 1α和TNF α的含量均明显高于正常对照组 (P <0 0 1)。糖尿病组中IL 1α和TNF α的含量在 3个不同时期差异无显著性 (P >0 0 5 )。结论 :糖尿病时 ,椎间盘组织中IL 1α和TNF α的含量明显增加 ,并维持在较高水平 。

Objective:To measure the content of the interleukin-1α (IL-1α)and tumor necrosis factor-α (TNF-α ) in the lumbar intervertebral discs of the diabetic rats for exploration of the possible reasons for the poor outcome in the diabetic patients who received lumbar discectomy.Method:Thirty-six male SD rats were randomly put into two groups,18 in each group.The diabetes mellitus (DM) was induced by a single intraperitoneal injection of streptozotocin(STZ) solution (40mg/kg).The rats were killed at 1^(st) month,3^(rd) months and 4^(th) months later respectively.Each time 6 diabetic rats and 6 control rats were sacrificed respectively with one lumbar intervertebral disc for the measurement of the content of the IL-1α and TNF-α by the enzyme-linked immunoadsorbent assay (ELISA).Result:At the three time points,the levels of the IL-1α and TNF-α in the intervertebral discs of the diabetic rats were significantly higher than those of the control rats (P<0.01).No significant differences in the levels of the IL-1α and TNF-α were found among the three groups of control rats (F=1.079,P=0.365;F=0.498,P=0.617) and the three groups of diabetic rats (F=0.605,P=0.559;F=0.234,P=0.795).Conclusion:The levels of the IL-1α and TNF-α in the intervertebral discs of the diabetic rats rise significantly and maintain for a long period,which may be related to the poor results following lumbar discectomy on the diabetic patients.