反相高效液相色谱法同时测定家兔血清中阿魏酸和川芎嗪浓度

Simultaneous Determination of Ferulic Acid and Tetramethylpyrazine in Rabbit Serum by RP-HPLC

目的 :建立同时测定家兔血清中阿魏酸和川芎嗪浓度的方法。方法 :水浴法去蛋白后 ,采用反相高效液相色谱法 ,以甲醇 -水 (7∶18 ,加入1 %冰醋酸 )为流动相 ,以ODSC18 不锈钢柱 (4 6mm×250mm )为固定相 ,紫外检测波长为280nm。结果 :阿魏酸在30 2～70 8μg/ml浓度范围内线性关系良好 (r=0 9974) ;磷酸川芎嗪在10 1～50 7μg/ml浓度范围内线性关系良好 (r=0 9922) ,二者色谱分离良好。结论 :本方法具有快速、精确、简便、不引入杂质的优点 ,可为人血清中中药多种有效成分的测定提供实验依据。

OBJECTIVE:To establish a method for simultaneous determination of ferulic acid(FA)and TMP in serum sample.METHODS:After deprotein with boiling water-bath,RP-HPLC was performed on ODS C 18 column(250mm×4.6mm)with methanol-water(containing0.1%acetic acid)=8∶17as the mobile phase and UV detection at280nm.RESU LTS:The calibration curve of FA was linear within the range of30.2～70.8μg/ml,Y=57.51+413.12X,r=0.9974;the curve of TMP was linear within the range of10.1～50.7μg/ml,Y=41.32+308.64X,r=0.9922.CONCLUSION:Compared with the traditional extraction,the method is simple,rapid and sensitive,and can be used for simultaneous determination of the contents of active compositions in Chinese materia medica.