摘要
目的 :建立同时测定家兔血清中阿魏酸和川芎嗪浓度的方法。方法 :水浴法去蛋白后 ,采用反相高效液相色谱法 ,以甲醇 -水 (7∶18 ,加入1 %冰醋酸 )为流动相 ,以ODSC18 不锈钢柱 (4 6mm×250mm )为固定相 ,紫外检测波长为280nm。结果 :阿魏酸在30 2~70 8μg/ml浓度范围内线性关系良好 (r=0 9974) ;磷酸川芎嗪在10 1~50 7μg/ml浓度范围内线性关系良好 (r=0 9922) ,二者色谱分离良好。结论 :本方法具有快速、精确、简便、不引入杂质的优点 ,可为人血清中中药多种有效成分的测定提供实验依据。
OBJECTIVE:To establish a method for simultaneous determination of ferulic acid(FA)and TMP in serum sample.METHODS:After deprotein with boiling water-bath,RP-HPLC was performed on ODS C 18 column(250mm×4.6mm)with methanol-water(containing0.1%acetic acid)=8∶17as the mobile phase and UV detection at280nm.RESU LTS:The calibration curve of FA was linear within the range of30.2~70.8μg/ml,Y=57.51+413.12X,r=0.9974;the curve of TMP was linear within the range of10.1~50.7μg/ml,Y=41.32+308.64X,r=0.9922.CONCLUSION:Compared with the traditional extraction,the method is simple,rapid and sensitive,and can be used for simultaneous determination of the contents of active compositions in Chinese materia medica.
出处
《中国药房》
CAS
CSCD
2004年第8期467-469,共3页
China Pharmacy
关键词
阿魏酸
川芎嗪
血药浓度
反相高效液相色谱法
水浴法
ferulic acid
tetramethylpyrazine
serum concentration of drug
RP-HPLC
boiling water-bath