摘要
目的 :建立以反相高效液相色谱法测定氢氯噻嗪片含量的方法。方法 :以ZORBAXC18 为色谱柱 ,0 02mol/L磷酸盐缓冲液 -甲醇 (80∶20)为流动相 ,紫外检测波长为226nm ,柱温为室温 ,进样量为20μl。结果 :氢氯噻嗪在0 006~0 303mg/ml浓度范围内线性关系良好 (r=1 0000 ,n=5) ,平均回收率为100 1 % ,RSD=0 43 % (n=9)。结论 :该法准确度高、精密度好、简便快速 ,可作为氢氯噻嗪片的含量测定方法。
OBJECTIVE:To establish a RP-HPLC method for determination of the content of hydrochlorothiazide tablets.METHODS:Samples were determined by RP-HPLC on a ZORBAX SB-C 18 column with a mixture of phosphate buffer solution(pH=4.0)-methanol(80∶20)as mobile phase and UV detection at226nm.RESULTS:The calibration curves of hydrochlorothiazide was linear from0.006to0.303mg/ml(r=1.0000,n=5),the average recovery and relative standard deviation were100.1%and0.43%(n=9)respectively.CONCLUSION:The method is rapid,simple,accurate and precise.It can be used for assay of hydrochlorothiazide.
出处
《中国药房》
CAS
CSCD
2004年第8期497-498,共2页
China Pharmacy