hBMP-2转染自体兔骨髓基质细胞附和异体兔脱钙骨基质的成骨实验研究

Bone Repairing Study of hBMP-2 Transfected rMSCs Attached to DBM

目的 探讨人骨发生蛋白 2 (hBMP - 2 )转染兔自体骨髓基质细胞 (rMSCs)的骨生成诱导作用以及附和异体兔脱钙骨基质 (DBM )后的成骨效能。方法 取兔自体骨髓基质细胞培养扩增 ,用脂质体介导的方法转染人骨发生蛋白 2基因 ,将转染和未转染人骨发生蛋白 2基因的自体骨髓基质细胞附和于异体兔脱钙骨基质形成新的生物植骨材料 ,连同空白及单纯脱钙骨基质对照组植入兔前肢肌袋和桡骨缺损。 2、 4、 6、 8周分别取材进行大体、放射线和病理观察。结果 肌袋试验 6周后 ,转染人骨发生蛋白 2基因的自体骨髓基质细胞附和异体兔脱钙骨基质材料中出现骨组织细胞 ,未转染组和单纯异体兔脱钙骨基质组为纤维组织。骨缺损试验中 ,三组均能产生骨的形成和缺损的修复 ,但仍然是转染复合材料组的骨修复再生能力最大。结论 局部应用hBMP -

Objective To detect the effect of hBMP-2 transfected rMSCs on bone repair and the capability of the new biomaterial in enhancing bone repair.Methods Auto-rMSCs were cultured and transfected with hBMP-2 by liposome. All the transfected and un-transfected rMSCs were attached to Allo-DBMs. These new biomaterials were implanted in muscle bags and segmental lone bone defects of the New Zealand white rabbits, and some controlled material groups were established for comparison. All the biomaterials and the controlled materials were assessed by gross observation, radiographical and histological methods.Results After six weeks, the oeteoblasts could be seen in the biomaterials with transfected rMSCs, which been implanted in muscle bags, there was no sign of bone formation in the biomaterials with untransfected rMSCs and the single DBM groups. With the long bone defects, all the transfected, untransfected and single DBM biomaterials could work, but the healing of the biomaterial with transfected hBMP-2 group was the fastest and most effective.Conclusion Delivery of rMSCs with transfected hBMP-2 gene may generate osteo-morphogenesis and promote skeletal repair in vivo.