鸢尾中鸢尾酮的鉴定及含量测定

Analysis and determination of irone in Iris tectorum

目的 建立鸢尾中鸢尾酮的鉴定和分析方法。方法 采用气相色谱 质谱法鉴定了鸢尾酮的 3种异构体 ,并采用气相色谱法测定了鸢尾浸膏中鸢尾酮的含量。结果 根据质谱裂解规律鉴定了 3种鸢尾酮的异构体 :α 、β 、γ 鸢尾酮 ,并且鸢尾浸膏中主要含γ 鸢尾酮。两批鸢尾中鸢尾酮的质量分数分别为 6 87、2 38μg/ g (n =6 ) ,质量分数差异较大。结论 采用气相色谱 质谱法和气相色谱法分析鸢尾中鸢尾酮 ,方法高效、准确 ,分析时间适中。

Object To establish an analysis method for determ in ation of irone in Iris tectorum Maxim. f. alba Makino. Methods The three isomers of irone were quantitatively analyz ed by GC-MS. Irone in I. tectorum extract was determinated by GC. Results The three isomers of irone, α, β, γ- irone were s p eculated according to the MS splitting decomposition law. Irone contents in the extract were 687, 238 μg/g (n=6), which h ad much difference. Conclusion The analysis method for irone by GC-MS and GC is hig her efficiency, precise, and the analysis time is acceptable.