摘要
目的 探讨大鼠衰老过程中阴茎组织中凋亡抑制基因 (bcl 2 )、凋亡促进基因 (bax)及阴茎组织成分等的变化与阴茎勃起的关系。 方法 以不同月龄 (2、8、16、2 4月 )大鼠为对象 ,进行了以下研究 :①阴茎组织Masson染色 ,显示平滑肌纤维、胶原纤维分布的变化 ;②应用免疫组化法检测阴茎组织中bcl 2、bax表达。 结果 ①随年龄增加阴茎组织中平滑肌纤维减少 ,胶原纤维增多 ;②大鼠阴茎组织中有bcl 2及bax表达 ,随年龄增加 ,成纤维细胞中bcl 2表达增强 ,bax表达减弱 ,而平滑肌细胞的变化正好相反。 结论 ①增龄对阴茎组织成分有明显影响 ,胶原纤维及平滑肌纤维的变化可能与勃起障碍的不可逆有关。②增龄的影响主要是引起平滑肌细胞的凋亡 ,而成纤维细胞则凋亡不明显 ,使阴茎组织中平滑肌细胞 /成纤维细胞失调。
Objective To study the effects of aging on expression of bcl-2,bax and components of rat's penile tissue. Methods Rats of different age(2,8,16,24 months) were studied as follows:1) smooth muscle fibers and collagen fibers were determined in penile tissue using Masson staining;2) the expression of bcl-2,bax in penile tissue was detected with immunohistochemical technique. Results ① With age increasing,smooth muscle fibers decreased while collagen fibers increased;② The expression of bcl-2 increased,while bax decreased in collagen cells.The changes in smooth muscle cells were just reverse. Conclusions ① The effect of aging on components of penile tissue is significant and the alterations of collagen fibers and smooth muscle fibers are probably relevant to the irreversibility of erectile dysfunction;②Aging mainly results in apoptosis of smooth muscle cell,while the apoptosis of collagen cell is not significant,so the ratio of smooth muscle cell and collagen cell loses its balance.
出处
《实用老年医学》
CAS
2004年第4期188-190,共3页
Practical Geriatrics
基金
国家自然科学基金资助项目 (30 0 0 0 0 63)
