槐耳清膏治疗肝癌的实验研究

Experimental research of the Fungi of Huai Er in treating liver cancer

目的 探讨槐耳清膏对人胎脐静脉内皮细胞的作用 ,以及其对裸鼠皮下接种肝癌细胞形成肝癌的抑制作用及其可能机制。方法 用不同浓度的槐耳清膏作用于人胎脐静脉血管内皮细胞 ,观察其对细胞的增殖能力、迁移能力、附壁能力及血管生成的影响 ,同时观察其对裸鼠皮下接种肝癌细胞形成肝癌过程的影响。结果 槐耳清膏≥ 2mg/ml时可显著降低肿瘤组织的血管内皮细胞增殖能力 ,减少血管形成 ,抑制血管内皮细胞的迁移、黏附 ,从而降低微血管密度。槐耳清膏 ( 3g/kg) +MMC( 5 0 0 μg/kg)同时作用于裸鼠的肝癌组织 ,其抑瘤作用最强 ,后依次为MMC组 ,槐耳清膏 ( 3g/kg)组。结论 槐耳清膏对肝癌有抑制作用 ,其可能机制是作用于血管内皮细胞 ,影响血管内皮细胞的增殖能力、迁移能力、附壁能力及血管生成 ,从而抑制肝癌组织的血管生成 。

Objective To study the effect of Fungi of Huai Er on human umbilicus vein endothelial cell((HUVEC)) and its inhibiting effect on liver cancer formation by liver cancer cells implanted under nude mice(subcutaneous) tissue, and to study the possible mechanism. Methods HUVEC was cultured with different concentrations of fungi of Huai Er and/or mitomycin(MMC) to observe the effect of Fungi of Huai Er on(HUVEC)′s hyperplasia , motility and adherence ability, and on the generation of blood vessels.In addition,the effect the Fungi of Huai Er on liver(cancer) formation by liver cancer cells implanted in nude mice(subcutaneous) tissue was also observed. Results Fungi ≥2mg/ml, can apparently reduce the hyperplasia(ability),inhibit motility and adherence ability of HUVEC,and the formation of blood vessels. The strongest effect on inhibiting the subcutaneous tumor formation was Fungi(3g/kg)plus MMC(500μg/kg),followed by MMC,and Fungi(3g/kg). Conclusions Possible mechanism of the inhibiting effect of Fungi on liver(cancer) is that the Fungi inhibits the(hyperplatic) ability, motility and adherence ability of HUVEC, and this(inhibits) the growth of blood vessels. Therefore, the growth of blood vessels is reduced and the development of liver cancer is suppressed.