hTERT mRNA和MDR mRNA在人肝癌中的表达及其意义

Expression and significance of hTERT mRNA and MDR mRNA in human primary hepatocellular carcinoma

目的 探讨hTRTmRNA及MDRmRNA在人肝细胞肝癌 (HCC )组织中的表达及其意义。方法 检测 5 8例HCC患者癌组织及癌旁组织中hTERTmRNA和MDRmRNA的表达情况。结果 在 5 8例肝癌患者癌组织及其癌旁组织中 ,hTERTmRNA阳性率分别为 44 .83 % ( 2 6/5 8)和 5 .17% ( 3 /5 8) ,差异有显著性 (P <0 .0 1) ;MDRmRNA阳性率分别为 60 .3 4% ( 3 5 /5 8)和 3 2 .76% ( 19/5 8) ,差异有显著性 (P <0 .0 1)。hTRTmRNA ,MDRmRNA在人肝癌组织中的检出率与临床分期、肝外转移、术后复发、肿瘤大小等明显相关 (均P <0 .0 5 ) ,而与肿瘤数目、血清AFP水平等无明显相关 (P >0 .0 5 )。MDRmRNA检出率与术前化疗明显相关 (P <0 .0 1) ,但与门静脉癌栓无关 (P >0 .0 5 ) ;而hTERTmRNA与术前化疗无相关 (P >0 .0 5 ) ,但与门静脉癌栓有关 (P <0 .0 5 )。结论 hTERTmRNA和MDRmRNA可能与肝细胞的恶性转化及术后复发有关 ,故可望作为预测肝癌复发。

Objective To evaluate the significance of telomerase(reverse) transcriptase (hTERT) and multidrug(resistance) (MDR) genes in human hepatocellular carcinoma (HCC). Methods Reverse transcription-polymerase chain reaction (RT PCR) method was employed to detect the hTERT mRNA and MDR mRNA in cancer tissues and para tumor liver tissues in 58 paticats with HCC. Results The expression of hTERT mRNA in HCC tissues (26/58, 44.83%) was significantly higher than that in para tumor liver tissues (3/58, 5.17%),(P<0.01). The (expression) of MDR mRNA in HCC tissues (35/58, 60.34%) was significantly higher than that in para-tumor liver tissues (19/58, 32.76%, P<0.01). The expressions of hTERT mRNA and MDR mRNA in the HCC tissue were correlated with the clinical stage, size of tumor, presence of extrahepatic metastasis, portal vein thrombosis and(recurrence) of HCC; but not(correlated) with the number of tumor, level of serum AFP. The use of the chemotherapy may increase the level of MDR mRNA, but did not affect the level of hTERT mRNA. Conclusions hTERT and MDR genes may relate to the hepatic cell malignant transformation and the recurrence of HCC. They might be(valuable) markers for predicting metastasis and recurrence of HCC, and as target genes for the treatment of HCC.