肝癌细胞凋亡端粒酶活性和bcl-2基因表达

Expression of changes in tissue apoptosis,telomerase and bcl-2 in the hapatocellular carcinoma(HCC)

目的 探讨经肝动脉化疗栓塞 (TACE)后bcl 2蛋白在肝癌细胞凋亡中的作用。方法 应用免疫组织化学法和原位末端标记法 (ISEL)检测 6 5例原发性肝癌患者分组治疗前后bcl 2蛋白表达和肝癌细胞凋亡。结果 肝癌 (HCC)患者不同治疗方法肝细胞凋亡阳性率和bcl 2阳性率均低于对照组 (P <0 0 5～0 0 1) ,端粒酶阳性率高于对照组 (P <0 0 5 ) ;手术 +化疗组的有效率和生存率均高于其他两组 ;中晚期肝癌生存率低于早期肝癌 (P <0 0 5～ 0 0 1) ;直径≥ 10cm中晚期肝癌生存率低于直径 <10cm中晚期肝癌 (P <0 0 5～ 0 0 1)。结论 细胞凋亡、端粒酶活性和bcl 2基因表达在肝癌发展过程中有重要作用。

Objective To investigate the changes in tissue apoptosis,telomerase and bcl 2 in the hepatocellular carcinoma(HCC) by transcatherter arterial chemoem boliztion(TACE) operation.Methods The expression of bcl 2 in HCC tissues was detected by immunohistochemical method and apoptosis by ISEL method in the 65 patients with hepatocelluler carcinoma and 20 cases in the control group.Results Expression of positive rate of apoptosis and bcl 2 in HCC was lower than that in the control group by different methods.The rate of efficiency and survival in surgical operation+chemotherapy group was higher than that in the other two group.The survival rates of metaphase and terminal were lower than that of forepart in HCC.The survival rate of diameter ≥10cm was lower than that of diameter <10cm.Conclusion The changes in tissue apoptosis,telomerase and bcl 2 have important function in patients with HCC.