富含CpG的脱氧核苷酸片段对破骨细胞形成的调节作用

CpG oligonucleotides in mediating osteoclast differentiation

目的 :研究富含CpG的脱氧核苷酸片段 (ODN)调节破骨细胞 (OC)的形成 .方法 :分离小白鼠骨髓单核细胞 ,单纯骨髓单核细胞或骨髓单核细胞分别与 30 μg/L CSF ;30 μg/L CSF ,2 0 μg/LRANKL ;30 μg/LM CSF以及不同浓度ODN共同培养 1～ 4d .结果 :ODN可以诱导骨髓单核细胞分化为TRAP阳性的破骨细胞 ,这些细胞表达降钙素受体 .Northernblot显示 :随着ODN浓度由 5nmol/L增加至 1 0 0nmol/L ,细胞TNF αmRNA表达量也随之增加 ;骨髓单核细胞与 30 μg/LM CSF ,2 0nmol/LODN 1 82 6共同培养 1 ,2 ,3和 4d ,同样显示随着时间延长 ,细胞TNF α表达也逐渐增加 .结论 :ODN可以刺激破骨细胞的转化 ,为临床骨疾病的治疗开辟了一个新途径 .

AIM: CpG oligonucleotides were studied in their mediation of osteoclast differentiation. METHODS: Mouse bone marrow mononuclear cells were separated and simple bone marrow mononuclear cells or bone marrow mononuclear cells were incubated separately with 30 μg/L M CSF, 30 μg/L M CSF and 20 μg/L RANKL or with 30 μg/L M CSF and CpG ODN of different concentrations for 1-4 d. RESULTS: CpG ODN induced bone marrow mononuclear cells to differentiate into TRAP positive osteoclasts and these cells expressed calcitonin receptors. Northern blot showed that the expression of TNF α mRNA increased with the increase of ODN from 5 nmol/L to 100 mol/L and it also increased gradually with prolonging time. CONCLUSION: CpG ODN stimulates the transformation of osteoclast, thus CpG ODN providing a potential therapeutic approach in treating bone diseases.