融合蛋白IFN-α2b-NGR在荷瘤小鼠体内的肿瘤局部分布及组织定位

Distribution in tumor location and tissue position of fusion protein IFN-α2b-NGR in tumor-bearing murine

目的 :研究融合蛋白IFN α2b NGR在荷瘤小鼠不同组织中的分布 .方法 :接种小鼠多发性骨髓瘤细胞株SP2 / 0于小鼠腋窝皮下 ,待肿瘤模型建立后 ,经小鼠尾静脉注射IFN α2b NGR ,IFN α2b和生理盐水 ,30min后取小鼠血清及组织匀浆上清 .用ELISA方法检测血清和组织上清中的IFN α浓度 ,免疫组化方法观察受试样品在组织中的分布情况 .结果 :ELISA检测结果表明 ,生理盐水组的小鼠血清及组织匀浆中均未检出IFN α ;IFN α2b组的血清IFN α浓度为(2 5 0± 6 5 )ng/L ,但在各种组织匀浆中均未检出 .IFN α2b NGR组的血清IFN α浓度为 (2 5 5± 6 1 )ng/L ;肿瘤组织匀浆中的浓度为 (1 91± 71 )ng/L ,血清和肿瘤组织中IFN α含量之比为 1 0 0∶75 ;其他组织匀浆中未检出IFN α2b .免疫组化结果表明IFN α2b NGR分布于肿瘤血管内壁表面及组织间隙中 .结论 :融合蛋白IFN α2b NGR具有对肿瘤组织的特异性结合能力 。

AIM: To study the distribution of fusion protein IFN α2b NGR in different tissues of a murine tumor model. METHODS: Tumor models were treated by intravenous injection of IFN α2b or IFN α2b NGR solutions and the concentration of IFN was measured using ELISA. The distribution was characterized by immunohistochemical methods. RESULTS: In the group of IFN α2b, the concentration of IFN in the murine serum was (250±65) ng/L and it was lower than 78 ng/L in tumor tissues. The concentration of IFN was (255±61) ng/L in serum and (191±70) ng/L in tumor tissues of the group of IFN α2b NGR. Immunohistochemical assay proved the binding of IFN α2b NGR to tumor vasculature walls and tissue spaces. CONCLUSION: Fusion protein IFN α2b NGR can be targeted to tumor and the binding sites are on vascular walls.