BLU和ARF基因在鼻NK/T细胞淋巴瘤中的甲基化状态比较

Comparison of hypermethylation of BLU and ARF genes in nasal NK/T cell lymphomas

目的 :检测BLU与ARF基因启动子在鼻NK/T细胞淋巴瘤中的甲基化状态 ,探讨其在肿瘤发生中的作用与分子病理诊断中的应用价值 .方法 :选用 1 2例已经确诊的鼻NK/T细胞淋巴瘤的冰冻组织标本和 35例石蜡组织标本 .将提取的DNA经亚硫酸盐处理后 ,用甲基化特异性PCR(methylationspecificPCR ,MSP)方法检测BLU和ARF基因启动子的甲基化状态 .结果 :在 1 2例鼻NK/T细胞淋巴瘤冰冻组织病例中 ,1 2例均有BLU基因高度甲基化 ,仅 1例有ARF基因高度甲基化 ;35例石蜡组织病例中 ,BLU基因高度甲基化 8例 ,ARF基因高度甲基化 3例 .BLU基因高度甲基化阳性率明显高于ARF基因 .结论 :BLU基因高度甲基化可能在鼻NK/T细胞淋巴瘤发生发展有重要作用 。

AIM: To investigate the hypermethylation of the BLU and ARF promoter in nasal NK/T cell lymphoma from nasopharyngeal mucosa and its roles in tumor genesis and molecular pathological diagnosis. METHODS: Forty seven cases of nasal NK/T cell lymphomas were studied by using methylation specific PCR (MSP). DNA was extracted from 12 frozen tissues and 35 paraffin embedded tissues, then treated by sodium bisulfite and PCR amplified by specific primers for methylated versus unmethylated DNA. PCR products were visualized under UV after agarose electrophresis. RESULTS: The hypermethylation of BLU gene promoter was detected in all the 12 frozen tissue cases (100%) and 8 out of 35 paraffine embedded tissue cases (23%) by methylated primer and the hypermethylation of ARF was observed in 1 of 20 frozen cases and 3 of 35 paraffine embedded cases. The hypermethylation of BLU was more frequent than that of ARF in all the cases. CONCLUSION: These findings suggest that hypermethylation of BLU gene may play a more substantial role than that of ARF gene in molecular mechanism of NK/T cell lymphoma. The detection of BLU methylation can be used as a molecular marker in pathological diagnosis.