Spantide抑制甲醛炎性痛大鼠脊髓一氧化氮合酶表达和一氧化氮含量的增加(英文)

Spantide prevents up-regulation of nitric oxide synthase expression and nitric oxide production in the spinal cord induced by fomaldehyde in rats

目的 观察鞘内注射P物质 (SP)拮抗剂spantide { [D Arg1,D Trp7,9,Leu11] substanceP}对炎性痛大鼠L5节段脊髓后角一氧化氮合酶 (NOS)表达和腰膨大一氧化氮 (NO)含量的影响 ,以探讨痛及痛过敏时脊髓NOS表达和NO生成增多的机制。方法 大鼠右后掌足底皮下注射 5 %甲醛 0 .2mL诱发炎性痛及痛过敏 ,NADPH d组化法观察脊髓后角NOS表达的变化 ,硝酸还原酶法测定NO含量的变化。结果 皮下注射甲醛 2 4h后 ,双侧L5节段脊髓后角NOS表达及腰膨大部位NO生成明显增加 ;注射甲醛前 5min鞘内注射spantide (5 μg ,10 μL) ,则明显抑制甲醛所致的NOS表达及NO生成增加。

AIM To study the effect of spantide {D Arg 1, D Trp 7,9 , Leu 11 ] substance P}, an antagonist of substance P (SP), on the up regulation of nitric oxide synthase (NOS) and nitric oxide (NO) in the spinal cord of rat during inflammatory pain and hyperalgesia. substance P}, an antagonist of substance P (SP), on the up regulation of nitric oxide synthase (NOS) and nitric oxide (NO) in the spinal cord of rat during inflammatory pain and hyperalgesia. METHODS The inflammatory pain and hyperalgesia were induced by subcutaneous injection of 5% fomaldehyde 0.2 mL into the right hind paw of the rats. NOS expression level was determined using NADPH diaphorase histochemistry, and NO production was determined by nitrate reductase method. RESULTS The fomaldehyde injection significantly increased NOS expression level and NO production in the dorsal horn of the L 5 segment of the spinal cord following pain and hyperalgesia. The increases reached its peak 24 h after the injection. Intrathecal injection of spantide (5 μg, 10 μL) 5 min prior to the fomaldehyde injection substantially inhibited the increases in NOS expression and NO production. CONCLUSION The release of SP resulted from nociceptive afferents plays an important role in the increases in NOS expression and NO production in the spinal cord of rat with pain and hyperalgesia induced by the fomaldehyde injection.