精密肝切片技术研究羟磷灰石纳米粒子对肝细胞的影响

Precision-cut rat liver slices used in studying effect of hydroxyapatite nanoparticles on the rat liver

目的 研究羟磷灰石纳米粒子 (HAP)对肝细胞是否具有直接的毒害作用。方法 以MTT还原量反映肝切片的存活度 ,乳酸脱氢酶漏出量 ,GPT及GOT漏出率反映对肝切片的毒性作用 ,以Na+ K+ ATP酶活性 ,蛋白质和糖原含量观察其代谢能力 ,以谷胱甘肽S 转移酶观察肝切片抗氧化水平的改变。结果 与对照组相比 ,0 .7和 1.4mmol·L- 1HAP可使肝切片谷胱甘肽S 转移酶活性 3～ 7h明显升高外 ,其他肝切片活性及代谢指标均无明显差异。结论 短期内HAP对肝切片无明显毒性作用 ,不表现浓度效应差异 ,可使肝细胞抗氧化酶活性升高。

AIM To study the direct damage of hydroxyapatite nanoparticles(HAP) to rat liver cells. METHODS MTT reduction was used to determine the survival of liver cells; GPT, GOT leakage (% of total activity) and LDH released in the medium were measured to reflect the toxicity to slices; the metabolic functions were studied by determination of Na + K + ATP activity and protein, glycogen content; the change of antioxidation was evaluated by GST activity. RESULTS Compared with the control, exposure of slices to HAP at different concentrations(0.7 and 1.4 mmol·L -1 )in the same media for 7 h showed no obvious differences in above criteria, except that GST activity of slices was significantly higher than that of control from 3 to 7 h. CONCLUSION In short period of time, there is no obvious noxiousness and no obvious concentration dependent effects of HAP on liver slices, but it could induce antioxidative enzyme activity of slices.