三氯化铝对大鼠海马CA1区锥体细胞钠电流的影响

Effects of aluminum chloride on sodium currents in acutely isolated rat hippocampal CA1 neurons

目的 为了进一步明确铝及含铝化合物对神经系统的损伤及其作用机制。方法 采用全细胞膜片钳技术研究三氯化铝 (AlCl3)对急性分离的大鼠海马CA1区神经元钠通道的影响。结果 AlCl3对钠电流有明显的抑制作用 ,且呈浓度依赖性 ,10 0 0μmol·L- 1AlCl3给药前后钠电流激活曲线Vh 分别为- (5 1.3± 6 .0 )mV和 - (4 7.5± 5 .4 )mV (P <0 .0 5 ) ,k值分别为 - (8.1± 2 .3)mV和 - (8.6± 3.2 )mV(P >0 .0 5 ) ,给药前后钠电流失活曲线Vh 分别为- (6 7.4± 5 .5 )mV和 - (71.4± 4 .4 )mV(P <0 .0 5 ) ,k值分别为 (6 .1± 1.1)mV和 (6 .8± 1.2 )mV (P >0 .0 5 )。结论 AlCl3对大鼠海马CA1区神经元钠通道有抑制作用 。

AIM To further explore the damage effect of aluminum and compounds containing aluminum on nervous system and its mechanisms. METHODS The effects of aluminum chloride (AlCl 3) on Na + currents of isolated hippocampal CA1 neurons of rats were studied using whole cell patch clamp technique. RESULTS AlCl 3 could reduce Na + current in a concentration dependent manner. Before and after application of 1000 μmol·L -1 AlCl 3, the half activation voltage of Na + current were -(51.3±6.0)mV and -(47.5±5.4) mV (P<0.05), but the slope was not changed. Before and after application of 1000 μmol·L -1 AlCl 3, the half inactivation voltage of Na + currents were -(67.4±5.5)mV and -(71.4±4.4)mV(P<0.05), but the slope was not changed. CONCLUSION AlCl 3 may damage sodium channels of the hippocampal CA1 neurons of rats, which may be related to the mechanism of damage of aluminum on central nervous system.