摘要
目的 观察“醒脑开窍”针法对局灶性脑缺血大鼠脑组织及血清 IL - 1β含量的影响。方法 以热凝法阻断一侧大脑中动脉造成大鼠局灶性脑缺血模型 ,采用放免法测定大鼠脑组织及血清 IL - 1β含量。结果 脑缺血后 3- 4 8h,脑组织 IL - 1β含量均较正常组和同时段假手术组显著升高 (P<0 .0 1) ,6 h达高峰 ;针刺治疗后 ,6 h到 4 8h脑组织 IL - 1β含量较同时段缺血组显著降低 (P<0 .0 1) ,但仍明显高于同时段假手术组和正常组 (P<0 .0 1或 P<0 .0 5 )。各组动物血清 IL - 1β含量无明显改变。结论 “醒脑开窍”针法能抑制缺血区脑组织 IL - 1β的合成和分泌 ,从而减轻由 IL - 1β引发的一系列脑缺血损害 ,发挥脑保护作用。
Objective To investigate the influence of brain-activating acupuncture on the IL-1β content of brain tissues and serum in rats with cerebral focal ischemia. Methods Unilateral cerebral middle artery was blocked by thermocoagulation and the IL-1 content of brain tissues and serum was determined by radioimmunoassay. Results The IL-1β content of brain tissues was significantly higher 3-48 hours after cerebral ischemia than in the normal group and than during the same period in the sham operation group (P<0.01) and reached the peak at the 6th hour; it was significantly lower 6-48 hours after acupuncture than during the same period in the ischemia group(P<0.01) but still significantly higher than during the same period in the sham operation group and than in the normal group(P<0.01 or P<0.05). The IL-1β content of serum did not significantly change in each group of animals. Conclusion Brain-activating acupuncture can inhibit the synthesis and secretion of IL-1β by ischemic brain tissues so as to reduce IL-1β-caused injury in cerebral ischemia and produce a protective effect on the brain.
出处
《上海针灸杂志》
2004年第8期35-37,共3页
Shanghai Journal of Acupuncture and Moxibustion
