不对称性多囊卵巢的卵巢局部受体异常的探讨

Defect of the Local Receptor in Asymmetric Polycystic Ovaries

目的 :探讨雄激素受体 (AR)和胰岛素受体 (INSR)在不对称性多囊卵巢 (PCO)局部发病机制中的作用。方法 :收集不对称性PCO 38例 ,应用ElivisionIM二步免疫组化法分别检测双侧卵巢细胞上AR和 β INSR的表达。结果 :在卵巢间质细胞上 ,PCO侧AR和β INSR的阳性表达为 16例 (4 2 .1% )和 2 2例 (5 7.9% ) ,明显少于非PCO侧AR的阳性表达38例 (10 0 % ,P <0 .0 1) ,明显多于非PCO侧 β INSR的阳性表达 2例 (5 .3% ,P <0 0 1) ;在卵泡内膜细胞上 ,PCO组AR和β INSR的阳性表达为 2 9例 (76 .3% )和 9例 (2 3.7% ) ,明显多于非PCO侧AR的阳性表达 12例 (31.6 % ,P <0 .0 1) ,明显少于非PCO侧 β INSR的阳性表达 2 0例 (5 2 .6 % ,P <0 .0 1) ;PCO发生在右侧者 2 8例 (73.7% ) ,明显多于左侧 10例(2 6 .3% ,P <0 .0 1)。结论 :卵巢局部AR和INSR信号表达异常与PCO的发生有关 ;

Objective:To probe the role of androgen receptor ( AR ) and insulin receptor ( INSR ) in pathogenesis of asymmetric polycystic ovaries ( PCO ) as local factors.Methods:38 patients of asymmetric PCO confirmed by pathology from January of 1998 to October of 2002 were enrolled . Ovaries of asymmetric PCO were divided into 2 groups: ovary with PCO as PCO group (n=38) and the other normal ovary side as non-PCO group (n=38) , The expression of AR and β-INSR of stroma cells , inner thecal cells and granulose cells were detected by immunohistochemistry .Results:The positive expression of AR stroma cells and inner thecal cells in PCO group and non-PCO group were 16 (42.1%) and 38 (100%) , 29 (76.3%) and 12(31.6%) respectively (both P<0 01); the positive expression of β-INSR in stroma cells and inner thecal cells in PCO group and non-PCO group were 22 (57.9%) and 2 (5.3%) , 9(23.7%) and 20(52.6%) respectively (both P<0 01);The case of PCO in right side was 28 (73.7%),which was higher than the left side 10(26.3%),(P<0 01).Conclusions:The lower expression of AR and higher expression of β-INSR in stroma cells,the higher expression and lower expression in inner thecal cell inside the local ovaries of asymmetric PCO play a role in the development of PCO ; The asymmetric PCO mostly appears at the right side of human body .